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kinB [Mon Aug 31 2015 13:56:31 GMT+0200 (CEST)]
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kinB [Mon Aug 31 2015 13:56:31 GMT+0200 (CEST)]

two-component sensor kinase, phosphorylates Spo0F, part of the phosphorelay, senses changes in respiratory activity
locus
BSU31450
pI
6.68
mw
47.00 kDa
function
initiation of sporulation
product
two-component sensor kinase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.1|The kinases]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.1|The kinases]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • [SW|Categories] containing this gene/protein

  • [SW|protein modification], [SW|transcription factors and their control], [SW|phosphorelay], [SW|membrane proteins], [SW|phosphoproteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|CodY regulon], [SW|stringent response]

    • The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [SW|Spo0F]
  • mainly active in the older, inner regions of a colony (with [protein|search|KinA]) [Pubmed|21097618]
  • sensing potassium level in the medium during initiation of [SW|sliding] [Pubmed|26152584]

    • [SW|Domains]

  • six transmembrane segments, C-terminal histidine phosphotransferase domain
  • selectivity filter sequence of potassium channels that is required for [SW|sliding] but not for [SW|sporulation] [Pubmed|26152584]

    • Modification

  • autophosphorylation on a His residue

    • Effectors of protein activity

  • activity is triggered at low respiratory activity, this depends on a functional interaction with the respiration apparatus [Pubmed|23599347]

    • Structure

  • [PDB|3D36] (from G. stearothermophilus, complex with [protein|search|Sda])

    • [SW|Localization]

  • cell membrane (integral membrane protein) [Pubmed|23599347]

    • [SW|Interactions]

  • [protein|search|KinB]-[protein|search|Sda]
  • [protein|search|KinB]-[SW|Spo0F]
  • [protein|search|KinB]-[protein|search|CtaC] [Pubmed|23599347]
  • [protein|search|KinB]-[protein|search|QcrC] [Pubmed|23599347]

    • Expression and Regulation

    Operon

  • ''[protein|search|kinB]-[protein|search|kapB]'' [Pubmed|8497199]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|8497199]

    • Regulation

  • repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455]
  • induced upon addition of decoyinine (positive [SW|stringent response]) [Pubmed|23378509]

    • Regulatory mechanism

  • [protein|search|CodY]: transcription repression [Pubmed|12618455]

    • Biological materials

    Mutant

  • JH19980 (''kinB''::''tet'') [Pubmed|9334321]

    • References

  • 26152584,8576055,16166384,9299348,8497199,10094672,9426145,11069677,12618455,11902725,12618455,7592498,21097618,19101565,23378509,23599347,26152584