ctaD

cytochrome-c oxidase (subunit I)

Locus: BSU_14900

Isoelectric point: 6.99

Molecular weight: 68.93 kDa

Protein length: 622 aa

Gene length: 1869 bp

Function: respiration

Product: cytochrome-c oxidase (subunit I)

Essential: no

E.C.: 1.9.3.1

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Electron transport and ATP synthesis → Respiration → Terminal oxidases
• Groups of genes → Efp-dependent proteins
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,561,569 1,563,437

Phenotypes of a mutant

• essential according to PubMed, non-essential according to PubMed

The protein

Catalyzed reaction/ biological activity

• 4 [Fe(II)cytochrome c] + 4 H⁺ + O₂ --> 4 [Fe(III)cytochrome c] + 2 H₂O (according to UniProt)

Protein family

• heme-copper respiratory oxidase family (with QoxB, according to UniProt)

Paralogous protein(s)

• QoxB

Cofactors

• 2 heme A molecules, Cu(B) PubMed

Structure

• 1FFT (the protein from E. coli, 44% identity) PubMed

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ctaB-ctaC-ctaD-ctaE-ctaF-ctaG
  Description: PubMed

  Regulatory mechanism

  • ResD: activation, PubMed, in ResD regulon
  • Abh: repression, synergistic repression with AbrB, in Abh regulon
  • CcpA: repression, in CcpA regulon
  • AbrB: repression, synergistic repression with Abh, in AbrB regulon

  Regulation

  • expressed under anaerobic conditions (ResD) PubMed

Additional information

• translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

Mutant

• BKE14900 (ctaD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGTGACCTCCCCCAAT, downstream forward: _UP4_GATGATAAAGGGGTGAAGGC
• BKK14900 (ctaD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGTGACCTCCCCCAAT, downstream forward: _UP4_GATGATAAAGGGGTGAAGGC

References

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