dacC

penicillin-binding protein 4A, D-alanyl-D-alanine carboxypeptidase

locus: BSU_18350

pI: 5.41

mw: 52.72 kDa

protein length: 491 aa

gene length: 1476 bp

function: carboxypeptidase

product: penicillin-binding protein 4A, D-alanyl-D-alanine carboxypeptidase

essential: no

synonyms: pbp

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 1,998,340 1,999,815

The protein

Catalyzed reaction/ biological activity

• cleavage ofD-Ala-D-Ala interpeptide bridges in peptidoglycan [Pubmed|19758330]
• Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine (according to UniProt)

Protein family

• peptidase S13 family (single member, according to UniProt)

Structure

• [PDB|1W5D] [pubmed|17582436]
• [PDB|2J9P] (complex with a peptidoglycan mimetic peptide) [pubmed|17582436]

[SW|Localization]

• during vegetative growth: distinct foci and bands at cell periphery [pubmed|14731276]
• secreted, may be anchored in the membrane via a C-terminal amphiphatic alpha helix [pubmed|9733705]

Expression and Regulation

Operons

• genes: [gene|993A863ED80BA5970C9145A35D407834B322C9DC|galM]-[gene|20A3120D2A45449745C9162EDCF386ED3163851B|dacC]
  description: [Pubmed|9733705]

  sigma factors

  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|9733705], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|10482513], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

Biological materials

Mutant

• MGNA-B075 (dacC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1074 NBRP B. subtilis, Japan]
• BKE18350 ([gene|20A3120D2A45449745C9162EDCF386ED3163851B|dacC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAATTTCCCCGCCTCTC, downstream forward: _UP4_TAATAGCGGGAGACCTGTTT
• BKK18350 ([gene|20A3120D2A45449745C9162EDCF386ED3163851B|dacC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAATTTCCCCGCCTCTC, downstream forward: _UP4_TAATAGCGGGAGACCTGTTT

GFP fusion

• 3102 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 ([gene|DE4857E8A228F145EFB9B7AD817585258344ED1F|amyE]::P_xyl-gfp-[gene|20A3120D2A45449745C9162EDCF386ED3163851B|dacC]1-1473 spc) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]
• 3104 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|20A3120D2A45449745C9162EDCF386ED3163851B|dacC]::pSG5044 (cat P_xyl-gfpa-[gene|20A3120D2A45449745C9162EDCF386ED3163851B|dacC]1-615) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]

References

Reviews

• 18266855

Original publications

• 9733705,10482513,19758330,14731276,17582436,9864321,21821766,22029692,23560856,26460848