aimP

Viral quorum sensor, Phage SPbeta lysogeny promoter

locus: BSU_20850

pI: 6.26

mw: 4.08 kDa

protein length: 41 aa

gene length: 126 bp

function: unknown

product: unknown

essential: no

synonyms: AimP,yopL

Genomic Context

categories

• [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.2|SP-beta prophage]

Gene

Coordinates: 2,208,855 2,208,980

The protein

Catalyzed reaction/ biological activity

• Proteolytically cleaved in extracellular space to yield hexapeptide (GMPRGA) regulator. Hexapeptide re-enters cells and binds to [protein|CAE7D8E45DA7CDE99D3EA114DF7E368A0A126389|AimR] to promote SPbeta lysogeny. ([Pubmed|31149347])

Structure

• Hexapeptide bound to [protein|CAE7D8E45DA7CDE99D3EA114DF7E368A0A126389|AimR]: [PDB|5Y24] ([Pubmed|30224798]), [PDB|5ZW6] ([Pubmed|30323253]), [PDB|6IM4] ([Pubmed|30421358]), [PDB|6HP5] ([Pubmed|30745087]), [PDB|6JG9] ([Pubmed|31149347])

Expression and Regulation

Operons

• genes: [gene|20735BFECA52826F0F4D55843EEE7798F414993C|aimP]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: transcription repression [Pubmed|12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

Biological materials

Mutant

• BKE20850 ([gene|20735BFECA52826F0F4D55843EEE7798F414993C|aimP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE20850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTCTCACCTCCTTTAA, downstream forward: _UP4_TAAAATCCATTGACACATAA
• BKK20850 ([gene|20735BFECA52826F0F4D55843EEE7798F414993C|aimP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK20850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTCTCACCTCCTTTAA, downstream forward: _UP4_TAAAATCCATTGACACATAA

References

• 12850135,12850135,20525796,30224798,30323253,30421358,30745087,31149347