acpS

acyl-carrier protein synthase, 4-phosphopantetheine transferase

locus: BSU_04620

pI: 9.91

mw: 13.58 kDa

protein length: 121 aa

gene length: 366 bp

function: fatty acid biosynthesis

product: acyl-carrier protein synthase

essential: yes

ec: 2.7.8.7

synonyms: ydcB

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 515,710 516,075

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] --> adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein] (according to UniProt)

Protein family

• P-Pant transferase superfamily (with [protein|1627167E7E9DBE444161A94D9F76317612C6401C|Sfp/2], according to UniProt)

[SW|Cofactors]

• magnesium [Pubmed|11489886]

Structure

• [PDB|1F7T] [Pubmed|10997907], [PDB|1F80] (complex with [protein|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|AcpA]) [Pubmed|10997907]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|200BE4802FCE3C860565B374EAADC9C1FEEF3E49|acpS]-[gene|CA71F577FDBB8C6E647AA56E16E4111651F47E17|ydcC]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C103 (ydcB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2101 NBRP B. subtilis, Japan]
• BKE04620 ([gene|200BE4802FCE3C860565B374EAADC9C1FEEF3E49|acpS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTATGATAACCTCCTTT, downstream forward: _UP4_TAGTCTGCATATTAGGGAAA

References

Reviews

• 15952903

Original Publications

• 11489886,15955059,10997907,11867633,30283133