SubtiBank SubtiBank
narI
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

narI

nitrate reductase (gamma subunit)
Locus
BSU_37250
Isoelectric point
10.04
Molecular weight
25.15 kDa
Protein length
223 aa Sequence Blast
Gene length
672 bp Sequence Blast
Function
nitrate respiration, nitrogen assimilation
Product
nitrate reductase (gamma subunit)
Essential
no
Synonyms

Genomic Context

      
Loading

Categories containing this gene/protein

Gene

Coordinates
3,823,558 3,824,229

The protein

Catalyzed reaction/ biological activity

  • quinol + nitrate --> quinone + H2O + nitrite (according to UniProt)
  • Cofactors

  • heme b PubMed
  • Structure

  • 1Q16 (the NarG-NarH-NarI complex of E. coli, 37% identity) PubMed
  • Localization

  • cell membrane (according to UniProt)
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • Fnr: activation, PubMed, in Fnr regulon
  • Regulation

  • expressed under anaerobic conditions (Fnr) PubMed
  • view in new tab

    Biological materials

    Mutant

  • BKE37250 (narI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATACCCCAGAGGATCTGCC, downstream forward: _UP4_TGAAAATCCCCCTTGCAAGA
  • BKK37250 (narI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATACCCCAGAGGATCTGCC, downstream forward: _UP4_TGAAAATCCCCCTTGCAAGA
  • References

    Reviews

    Loading

    Original publications

    Loading