lpdV [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

2-oxoisovalerate dehydrogenase (E3 subunit, dihydrolipoamide dehydrogenase)

locus: BSU24060

pI: 4.89

mw: 48.00 kDa

function: utilization of branched-chain keto acids

product: 2-oxoisovalerate dehydrogenase
(E3 subunit, dihydrolipoamide dehydrogenase)

essential: no

ec: 1.8.1.4

synonyms: yqiV, bkd

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]

[SW|Categories] containing this gene/protein

• [SW|utilization of amino acids]

This gene is a member of the following [SW|regulons]

• [SW|BkdR regulon], [SW|CodY regulon], [SW|SigL regulon]

Gene

Coordinates on the chromosome (coding sequence): 2,500,104 -> 2,501,528

The protein

Catalyzed reaction/ biological activity

• Protein N(6)-(dihydrolipoyl)lysine + NAD⁺ = protein N(6)-(lipoyl)lysine + NADH (according to Swiss-Prot)

Protein family

• class-I pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|search|AcoL], [protein|search|PdhD]

Structure

• [PDB|2YQU] (from ''Thermus thermophilus (hb8 mutant)'', 42% identity, 55% similarity)

Expression and Regulation

Operon

• ''[protein|search|ptb]-[protein|search|bcd]-[protein|search|buk]-[protein|search|lpdV]-[SW|bkdAA]-[SW|bkdAB]-[protein|search|bkdB]'' [Pubmed|12823818]

[SW|Sigma factor]

• [protein|search|SigL] [Pubmed|10094682]

Regulation

• induced in the presence of isoleucine or valine ([protein|search|BkdR]) [Pubmed|10094682]

Regulatory mechanism

• [protein|search|CodY]: transcription repression [Pubmed|10094682]
• [protein|search|BkdR]: transcription activation [Pubmed|10094682]

References

• 12427936,15241682,10094682,12823818