mraW

S-adenosyl-L-methionine-dependent methyltransferase

Locus: BSU_15140

Isoelectric point: 6.03

Molecular weight: 35.15 kDa

Protein length: 311 aa

Gene length: 936 bp

Function: unknown

Product: S-adenosyl-L-methionine-dependent methyltransferase

Essential: no

E.C.: 2.1.1.199

Synonyms: yllC,ylxA

Genomic Context

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Categories containing this gene/protein

• Groups of genes → Poorly characterized/ putative enzymes

Gene

Coordinates: 1,580,622 1,581,557

The protein

Catalyzed reaction/ biological activity

• cytidine¹⁴⁰² in 16S rRNA + S-adenosyl-L-methionine --> H⁺ + N⁴-methylcytidine¹⁴⁰² in 16S rRNA + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• Methyltransferase superfamily (according to UniProt)

Structure

• 1N2X (from Thermotoga maritima, 41% identity, 70% similarity) PubMed
• 3TKA (from Escherichia Coli, 46% identity)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: mraZ-mraW-ftsL-pbpB
  Description: PubMed

  Regulation

  • constitutively expressed PubMed

Additional information

• the mRNA is cleaved by RNase III PubMed

Biological materials

Mutant

• MGNA-B130 (ylxA::erm), available at the NBRP B. subtilis, Japan
• 1A818 ( mraW::erm), PubMed, available at BGSC
• BKE15140 (mraW::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTGTGTTGGTCCCACC, downstream forward: _UP4_TAACGAAACGCGAACGCAAA
• BKK15140 (mraW::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTGTGTTGGTCCCACC, downstream forward: _UP4_TAACGAAACGCGAACGCAAA

References

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