abnA [2019-07-02 09:53:19]

endo-1,5-alpha-L-arabinosidase

locus: BSU28810

pI: 8.62

mw: 34.13 kDa

protein length: 323 aa

gene length: 972 bp

function: arabinan degradation

product: endo-1,5-alpha-L-arabinosidase

essential: no

ec: 3.2.1.99

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.6|Utilization of arabinan/ arabinose/ arabitol]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 2,949,053 2,950,024

The protein

Catalyzed reaction/ biological activity

• Endohydrolysis of (15)-alpha-arabinofuranosidic linkages in (15)-arabinans (according to Swiss-Prot)

Protein family

• glycosyl hydrolase 43 family (according to Swiss-Prot)

Structure

• [PDB|1UV4]

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|1F85A1324B8694FB3731376AFE6FFB162C096586|abnA]
  description: [Pubmed|14973026]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|14973026], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|A567466894AE9DE7CDE7816615433A37532297B5|AraR]: repression, [Pubmed|14973026], in [regulon|A567466894AE9DE7CDE7816615433A37532297B5|AraR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|22900538], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • repressed in the absence of arabinose ([protein|search|AraR]) [Pubmed|14973026]

  additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

Biological materials

Mutant

• BKE28810 ([gene|1F85A1324B8694FB3731376AFE6FFB162C096586|abnA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTGTTCAACCTCCTT, downstream forward: _UP4_TGAATTAAAAAGCCGGGCTC
• BKK28810 ([gene|1F85A1324B8694FB3731376AFE6FFB162C096586|abnA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTGTTCAACCTCCTT, downstream forward: _UP4_TGAATTAAAAAGCCGGGCTC

References

• 15556708,14973026,12884008,22900538,14973026,18957862