proline dehydrogenase (L-proline, NAD)
function
proline utilization
product
proline dehydrogenase (L-proline, NAD)
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.2|Utilization of proline][SW|Categories] containing this gene/protein
[SW|utilization of amino acids], [SW|sporulation/ other]This gene is a member of the following [SW|regulons]
[SW|CodY regulon], [SW|PutR regulon], [SW|Spo0A regulon]Gene
Coordinates on the chromosome (coding sequence)
344,551 -> 345,462
Phenotypes of a mutant
no growth with proline as single source of carbon or nitrogen [Pubmed|21840319]inactivation of ''[SW|putB]'' eliminates sporulation [Pubmed|26735940]The protein
Catalyzed reaction/ biological activity
L-proline acceptor = (S)-1-pyrroline-5-carboxylate reduced acceptor (according to Swiss-Prot)Protein family
proline dehydrogenase family (according to Swiss-Prot)Paralogous protein(s)
[protein|search|FadM]Structure
[PDB|4H6Q] (from ''Deinococcus radiodurans '', 42% identity) [Pubmed|23151026]Expression and Regulation
Operon
''[protein|search|putB]-[protein|search|putC]-[protein|search|putP]'' [Pubmed|21840319, 22139509][SW|Sigma factor]
[protein|search|SigA] [Pubmed|21840319,21964733,22139509]Regulation
induced in the presence of proline ([protein|search|PutR]) [Pubmed|21840319,22139509]expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455]strongly induced in response to glucose starvation in M9 medium [Pubmed|23033921]Regulatory mechanism
[protein|search|PutR]: transcription activation [Pubmed|21840319,21964733,22139509][SW|Spo0A]: transcription activation [Pubmed|14651647][protein|search|CodY]: transcription repression, displacement of [protein|search|PutR] [Pubmed|21840319]Additional information
overexpressed in a '[protein|search|pnpA]' mutant [PubMed|14976255]References
14976255,21840319,14651647,12618455,23033921,21964733,22139509,23151026,26735940 
