pbpX [2018-02-16 18:08:11]

penicillin-binding protein X

locus: BSU16950

pI: 9.71

mw: 43.70 kDa

protein length: 391 aa

gene length: 1173 bp

function: endopeptidase

product: penicillin-binding protein X

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,765,859 → 1,767,034

Phenotypes of a mutant

• increased sensitivity to lysozyme [Pubmed|21856855]
• inactivation of ''[gene|1F2062E59607706FE26A22876544F91B3B13179E|pbpX]'' strongly restores beta-lactam resistance in a ''[gene|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]'' mutant [Pubmed|22211522]

The protein

Structure

• [PDB|3TG9] (a PBP from B. halodurans, aa 90-375, 28% identity)

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]
• localizes during sporulation to both asymmetric septa and the prespore [pubmed|15758244]

Expression and Regulation

Operons

• genes: [gene|1F2062E59607706FE26A22876544F91B3B13179E|pbpX]
  description: [Pubmed|14762009]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX]: sigma factor, [Pubmed|14762009,15758244], in [regulon|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX regulon]
  • [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV]: sigma factor, [Pubmed|21926231], in [regulon|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV regulon]

  regulation

  • expression is reduced in a [protein|search|SigV] mutant [Pubmed|21926231]

Biological materials

Mutant

• MGNA-B074 (pbpX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1073 NBRP B. subtilis, Japan]
• BKE16950 (Δ[gene|1F2062E59607706FE26A22876544F91B3B13179E|pbpX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTTTTTCCTCCCAA, downstream forward: _UP4_TGATTGGCGATCTTCTTTTG
• BKK16950 (Δ[gene|1F2062E59607706FE26A22876544F91B3B13179E|pbpX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTTTTTCCTCCCAA, downstream forward: _UP4_TGATTGGCGATCTTCTTTTG

GFP fusion

• 3107 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|1F2062E59607706FE26A22876544F91B3B13179E|pbpX]::pSG5047 (cat P_xyl-gfpa-[gene|1F2062E59607706FE26A22876544F91B3B13179E|pbpX]1-426) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]

References

• 14762009,18957862,18179421,21856855,21926231,22211522,14731276