SubtiBank SubtiBank
yncF
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yncF

dUTPase
locus
BSU_17660
pI
5.10
mw
16.27 kDa
protein length
144 aa Sequence Blast
gene length
435 bp Sequence Blast
function
nucleotide metabolism
product
dUTPase
essential
no
ec
3.6.1.23
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.4|Nucleotide metabolism/ other]

    • Gene

    Coordinates
    1,900,080 1,900,514

    The protein

    Catalyzed reaction/ biological activity

  • dUTP + H2O --> diphosphate + dUMP + H+ (according to UniProt)

    • Protein family

  • dUTPase family (with [protein|B004744E17773FDEAB28FEAE98BCA3C744E63A8E|YosS], according to UniProt)

    • Paralogous protein(s)

  • [protein|B004744E17773FDEAB28FEAE98BCA3C744E63A8E|YosS]

    • Structure

  • [PDB|2XCD] [Pubmed|23897460]
  • [PDB|2XCE] (complex with dUpnhpp) [Pubmed|23897460]

    • Biological materials

    Mutant

  • MGNA-B096 (yncF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1095 NBRP B. subtilis, Japan]
  • BKE17660 ([gene|1E32B355838787D34352674595A63355609D3AC4|yncF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTACTAGCTCCTTTAAT, downstream forward: _UP4_TAAACAAAATAAATCCTTGG
  • BKK17660 ([gene|1E32B355838787D34352674595A63355609D3AC4|yncF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTACTAGCTCCTTTAAT, downstream forward: _UP4_TAAACAAAATAAATCCTTGG

    • References

  • 19342774,20823546,22383849