accC [Wed Sep 23 2015 18:43:08 GMT+0200 (CEST)]

acetyl-CoA carboxylase (biotin carboxylase subunit)

Locus: BSU24340

Isoelectric point: 4.00

Molecular weight: 49.00 kDa

Function: production of malonyl-CoA, the substrate for fatty acid biosynthesis

Product: acetyl-CoA carboxylase (biotin carboxylase subunit)

Essential: yes

E.C.: 6.3.4.14

Synonyms: yqhX

Genomic Context

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Categories containing this gene/protein

• Metabolism → Lipid metabolism → Biosynthesis of lipids → Biosynthesis of fatty acids
• Groups of genes → Essential genes

Categories containing this gene/protein

• biosynthesis of lipids, essential genes

Gene

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ATP biotin-carboxyl-carrier protein CO₂ = ADP phosphate carboxybiotin-carboxyl-carrier protein (according to Swiss-Prot)

Paralogous protein(s)

• YotE

Structure

• 1BNC (the protein from E. coli) PubMed

Interactions

• (AccA-AccD)-AccB-AccC (acetyl-CoA carboxylase complex)

Expression and Regulation

Operon

• accB-accC-yqhY PubMed

Biological materials

Mutant

• GP1478 (chromosomal accC-Strep fusion, aphA3), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab

GFP fusion

• GP1491 (spc, based on pBP43), available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

FLAG-tag construct

• GP1483 (spc, based on pGP1331), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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