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accC
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accC

acetyl-CoA carboxylase (biotin carboxylase subunit)
locus
BSU_24340
pI
4.72
mw
49.41 kDa
protein length
450 aa Sequence Blast
gene length
1353 bp Sequence Blast
function
production of malonyl-CoA, the substrate for fatty acid biosynthesis
product
acetyl-CoA carboxylase (biotin carboxylase subunit)
essential
yes
ec
6.3.4.14
synonyms
yqhX
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    2,530,354 2,531,706

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP + hydrogencarbonate + N6-biotinyl-L-lysyl-[protein] --> ADP + H+ + N6-carboxybiotinyl-L-lysyl-[protein] + phosphate (according to UniProt)
  • acetyl-CoA + ATP + hydrogencarbonate --> ADP + H+ + malonyl-CoA + phosphate (according to UniProt)

    • Paralogous protein(s)

  • [protein|5C8815965146BCD8ED238258B3F820AA17241165|YotE]

    • [SW|Domains]

  • [SW|ATP-grasp domain] (aa 120-318) (according to UniProt)
  • Biotin carboxylation domain (aa 1-447) (according to UniProt)

    • Structure

  • [PDB|1BNC] (the protein from ''E. coli'') [Pubmed|7915138]

    • Expression and Regulation

    Operons

    genes
    [gene|ADD1CE87C1C5BA44FFF5499EA5E155C4C53A1C22|accB]-[gene|1DFFEB155028A5C6B4B344769EF7DAAC14983322|accC]-[gene|FACE7176B1A1F354CF27FB30E46A1140FAFB02F0|yqhY]
    description
    [Pubmed|7592499]

    regulation

  • expression of the operon is strongly reduced during [SW|biofilm formation] [pubmed|31113899]
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    Biological materials

    Expression vectors

  • GP1478 (chromosomal ''accC''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
  • pGP1025, N-terminal Strep-tag, based on [SW|pGP172], purification from ''E. coli'', available in [SW|Jörg Stülke]'s lab

    • GFP fusion

  • GP1491 (spc, based on [SW|pBP43]), available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1483 (''spc'', based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 15952903,12121720,15968460

    • Original Publications

  • 7592499,7915138,28579978,28516784