SubtiBank SubtiBank
yvrB [2019-06-12 10:23:04]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

yvrB [2019-06-12 10:23:04]

ABC transporter (ATP binding domain) for cobalamin
Locus
BSU33170
Isoelectric point
11.07
Molecular weight
37.34 kDa
Protein length
353 aa Sequence Blast
Gene length
1062 bp Sequence Blast
Function
uptake of cobalamin
Product
ABC transporter (ATP binding domain)
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
Loading

Categories containing this gene/protein

  • Cellular processes → Transporters → ABC transporters → Importers → Uptake of cofactors
  • Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis/ acquisition of cobalamine (B12))
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    3,402,469 3,403,530

    The protein

    Protein family

  • Binding-protein-dependent transport system permease family (according to UniProt)

    • Structure

  • 4R9U, the E. coli BtuC-BtuD complex, BtuC shares 40% identity, 71% similarity with YvrB, PubMed

    • Expression and Regulation

    Operons

    Genes
    yvrC-yvrB-yvrA-yvqK

    Regulatory mechanism

  • B12 riboswitch: termination, upon binding of B12 PubMed, in B12 riboswitch

    • Regulation

  • expression is decreased in the presence of cobalamin (vitamin B12) (B12 riboswitch) PubMed
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B044 (yvrB::erm), available at the NBRP B. subtilis, Japan
  • BKE33170 (yvrB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACCAATGATATATGCGGCAT, downstream forward: _UP4_CATCGCGGGGGGAGAAGCTT
  • BKK33170 (yvrB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACCAATGATATATGCGGCAT, downstream forward: _UP4_CATCGCGGGGGGAGAAGCTT

    • References

    Loading