rbsB

ribose [SW|ABC transporter] (binding protein)

locus: BSU_35960

pI: 6.14

mw: 32.07 kDa

protein length: 305 aa

gene length: 918 bp

function: ribose uptake

product: ribose [SW|ABC transporter] (binding protein)

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.1|Uptake of carbon sources]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.4|Utilization of ribose]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,706,145 3,707,062

The protein

Protein family

• bacterial solute-binding protein 2 family (single member, according to UniProt)

Structure

• [PDB|2GX6] (from ''Escherichia coli k12 mutant'', 54% identity, 71% similarity)

[SW|Localization]

• extracellular (signal peptide), lipid modification as retention signal [Pubmed|18957862]
• attached to the cell membrane (via [protein|F1258E31151E137A850811B0C851D473CEFFD43B|RbsC]-[protein|F0E6F191FAF8F279462B09BF1FE4C6C752A57803|RbsD]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|77AEAC0F06B4D5F062D4A2740794B7BFDDA31096|rbsR]-[gene|C229BD2C00B169CF4A99482E65B3F749AD9D51D8|rbsK]-[gene|F0E6F191FAF8F279462B09BF1FE4C6C752A57803|rbsD]-[gene|AB4A686F62550F90F5BD8D27DAADC9C33F1DA1DB|rbsA]-[gene|F1258E31151E137A850811B0C851D473CEFFD43B|rbsC]-[gene|1D67888E79B09956F9258F4068546D8C67103549|rbsB]
  description: [Pubmed|7921236]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7511775], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|7921236], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: activation, [Pubmed|7592460], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • expressed upon transition into the stationary phase ([protein|search|AbrB]) [Pubmed|7592460]

Biological materials

Mutant

• BKE35960 ([gene|1D67888E79B09956F9258F4068546D8C67103549|rbsB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAACCCTCCTAAGC, downstream forward: _UP4_TAATTGTCTGATGTTTAGGA
• BKK35960 ([gene|1D67888E79B09956F9258F4068546D8C67103549|rbsB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAACCCTCCTAAGC, downstream forward: _UP4_TAATTGTCTGATGTTTAGGA

References

• 10092453,16872404,7511775,7592460,18957862,7921236