srfAC

surfactin synthetase / competence

Locus: BSU_03510

Isoelectric point: 4.97

Molecular weight: 143.59 kDa

Protein length: 1275 aa

Gene length: 3828 bp

Function: antibiotic synthesis

Product: surfactin synthetase / competence

Essential: no

Synonyms: comL

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Miscellaneous metabolic pathways → Biosynthesis of antibacterial compounds
• Lifestyles → Exponential and early post-exponential lifestyles → Swarming
• Lifestyles → Coping with stress → Biosynthesis of antibacterial compounds
• Groups of genes → Membrane proteins
• Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

Gene

Coordinates: 398,532 402,359

The protein

Protein family

• ATP-dependent AMP-binding enzyme family (according to UniProt)

Paralogous protein(s)

• reiterated SrfAC-like proteins: DhbF, PpsA, PpsB, PpsC, PpsD, SrfAA, SrfAB
• partial match (more than 1,000 amino acids): PksJ, PksN

Domains

• Carrier domain (aa 968-1043) (according to UniProt)

Modification

• phosphorylation on Ser-1003 PubMed

Structure

• 2VSQ PubMed

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: srfAA-srfAB-comS-srfAC-srfAD
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • PhoP: activation, PubMed, in PhoP regulon
  • CodY: repression, PubMed, in CodY regulon
  • ComA: activation, PubMed, in ComA regulon
  • PerR: activation, PubMed, in PerR regulon
  • Spx: repression, PubMed, in Spx regulon
  • Abh: repression, PubMed, in Abh regulon

  Regulation

  • expressed at high cell density (ComA) PubMed

  Additional information

  • A ncRNA is predicted between 'hxlR' and 'srfAA' PubMed

Biological materials

Mutant

• BKE03510 (srfAC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGTTTCGCTCCCGCCT, downstream forward: _UP4_TAAATGAAGTGATGAAAGGA
• BKK03510 (srfAC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGTTTCGCTCCCGCCT, downstream forward: _UP4_TAAATGAAGTGATGAAAGGA

References

Reviews

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Research papers

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