SubtiBank SubtiBank
levR
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levR

transcriptional activator of the [gene|75C568C8F6912AC064B8B435975D52510D25466A|levD]-[gene|CAE38D8F25A5EC6AC8045E40072E6FEC632045FA|levE]-[gene|8ABA3735067C7AA433E09F219EE7239196377A95|levF]-[gene|F23B951F60A6A669D5BD86836906E0F4AD7A0C1B|levG]-[gene|search|sacC ]operon
locus
BSU_27080
pI
5.94
mw
105.99 kDa
protein length
935 aa Sequence Blast
gene length
2808 bp Sequence Blast
function
regulation of levan and fructose utilization
product
transcriptional activator, PRD-type (for [protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL]-dependent promoter)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.7|Utilization of fructose]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.3|PRD-type regulators]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    2,763,025 2,765,832

    The protein

    Catalyzed reaction/ biological activity

  • binding to the upstream activating sequence in front of the [gene|75C568C8F6912AC064B8B435975D52510D25466A|levD] gene results in activation of transcription of the [gene|75C568C8F6912AC064B8B435975D52510D25466A|levD]-[gene|CAE38D8F25A5EC6AC8045E40072E6FEC632045FA|levE]-[gene|8ABA3735067C7AA433E09F219EE7239196377A95|levF]-[gene|F23B951F60A6A669D5BD86836906E0F4AD7A0C1B|levG]-[gene|61D5B957E018D019AE0CE1D5F6C6A33EF982ED49|sacC] operon, bining occurs in the presence of fructose

    • Protein family

  • [SW|Transcription factors activating transcription at SigL-dependent promoters]
  • [SW|PRD-containing transcription factors] (according to UniProt)

    • [SW|Domains]

  • [SW|Sigma-54 factor interaction domain] (aa 117-348) (according to UniProt)
  • 2 [SW|PRD domain]s (aa 468-573, aa 831-935) (according to UniProt)
  • [SW|PTS EIIA domain] type-4 (aa 574-711) (according to UniProt)

    • Modification

  • phosphorylation (His503, His582, His866) (according to Swiss-Prot)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • additional information

  • information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000052 PRODORIC2 database]

    • Expression and Regulation

    Operons

    genes
    [gene|1D2043CC0D8CF64142F0A5993A936C5A196726D4|levR]
    description
    [pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • BKE27080 ([gene|1D2043CC0D8CF64142F0A5993A936C5A196726D4|levR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTCTATCATCCTTTGTC, downstream forward: _UP4_TAAAAACAGTTTTTTCATAT
  • BKK27080 ([gene|1D2043CC0D8CF64142F0A5993A936C5A196726D4|levR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTCTATCATCCTTTGTC, downstream forward: _UP4_TAAAAACAGTTTTTTCATAT

    • References

  • 1900939,7592487,7592486,8057358,9033408,21906631,9622354