ptb [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

phosphate butyryltransferase

locus: BSU24090

pI: 7.00

mw: 31.00 kDa

function: utilization of branched-chain keto acids

product: phosphate butyryltransferase

essential: no

ec: 2.3.1.19

synonyms: yqiS, bkd

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]

[SW|Categories] containing this gene/protein

• [SW|utilization of amino acids]

This gene is a member of the following [SW|regulons]

• [SW|BkdR regulon], [SW|CodY regulon], [SW|SigL regulon]

Gene

Coordinates on the chromosome (coding sequence): 2,503,765 -> 2,504,664

The protein

Catalyzed reaction/ biological activity

• Butanoyl-CoA phosphate = CoA butanoylphosphate (according to Swiss-Prot)

Protein family

• phosphate acetyltransferase and butyryltransferase family (according to Swiss-Prot)

Structure

• [PDB|1YCO] (from ''Enterococcus faecalis v583'', 36% identity, 56% similarity)

Expression and Regulation

Operon

• ''[protein|search|ptb]-[protein|search|bcd]-[protein|search|buk]-[protein|search|lpdV]-[SW|bkdAA]-[SW|bkdAB]-[protein|search|bkdB]'' [Pubmed|12823818]

[SW|Sigma factor]

• [protein|search|SigL] [Pubmed|10094682]

Regulation

• induced in the presence of isoleucine or valine ([protein|search|BkdR]) [Pubmed|10094682]

Regulatory mechanism

• [protein|search|CodY]: transcription repression [Pubmed|10094682]
• [protein|search|BkdR]: transcription activation [Pubmed|10094682]

Biological materials

Mutant

• MGNA-C417 (yqiS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2415 NBRP B. subtilis, Japan]

References

• 12427936,15241682,12823818,10094682