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ptb
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ptb

phosphate butyryltransferase
Locus
BSU_24090
Isoelectric point
7.94
Molecular weight
31.62 kDa
Protein length
299 aa Sequence Blast
Gene length
900 bp Sequence Blast
Function
utilization of branched-chain keto acids
Product
phosphate butyryltransferase
Essential
no
E.C.
2.3.1.19
Synonyms
yqiS,bkd
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of branched-chain amino acids

    • Gene

    Coordinates
    2,503,765 2,504,664

    The protein

    Catalyzed reaction/ biological activity

  • butanoyl-CoA + phosphate --> butanoyl phosphate + CoA (according to UniProt)

    • Protein family

  • phosphate acetyltransferase and butyryltransferase family (with Pta, according to UniProt)

    • Structure

  • 1YCO (from Enterococcus faecalis v583, 36% identity, 56% similarity)

    • Expression and Regulation

    Operons

    Genes
    ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB
    Description
    PubMed

    Sigma factors

  • SigL: sigma factor, PubMed, in SigL regulon

    • Regulatory mechanism

  • BkdR: activation, PubMed, in BkdR regulon
  • CodY: repression, PubMed, in CodY regulon

    • Regulation

  • induced in the presence of isoleucine or valine (BkdR) PubMed
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    Biological materials

    Mutant

  • MGNA-C417 (yqiS::erm), available at the NBRP B. subtilis, Japan
  • BKE24090 (ptb::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTGTTACCACCTTTCTT, downstream forward: _UP4_TACACACATTAGGAGGAACG
  • BKK24090 (ptb::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTGTTACCACCTTTCTT, downstream forward: _UP4_TACACACATTAGGAGGAACG

    • References

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