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mtlR [2019-07-23 09:16:21]
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mtlR [2019-07-23 09:16:21]

transcriptional activator of the mtlA-mtlF-mtlD operon
Locus
BSU_04160
Isoelectric point
5.31
Molecular weight
78.67 kDa
Protein length
694 aa Sequence Blast
Gene length
2085 bp Sequence Blast
Function
regulation of mannitol utilization
Product
transcriptional activator, PRD-type
Essential
no
Synonyms
ydaA

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
467,130 469,214

The protein

Protein family

Domains

  • N-terminal DNA-binding domains, two PTS-regulation domains (PRD1 and PRD2), EIIB (Gat)-like domain, EIIA (Mtl)-like domain
  • Modification

  • phosphorylated on His-342 and/or His-399 by PtsH PubMed
  • phosphorylation on His-599 by MtlA PubMed
  • phosphorylation on Cys-419 by MtlF PubMed
  • Effectors of protein activity

  • activity is stimulated by PtsH-dependent phosphorylation in PRD2 (mechnism of carbon catabolite repression) PubMed
  • activity is inhibited by MtlF-dependent phosphorylation in the EIIB(Gat)-like domain on Cys-419 (this prevents activity in the absence of mannitol and allows induction in presence of mannitol) PubMed
  • Localization

  • upon interaction with non-phosphorylated MtlA, the active MtlR localizes to the membrane PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • Regulation

  • repressed by glucose (CcpA) PubMed
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    Biological materials

    Mutant

  • BKE04160 (mtlR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAAAAAGACCTCCTAGC, downstream forward: _UP4_TAAACCTGCATGGCACACGT
  • BKK04160 (mtlR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAAAAAGACCTCCTAGC, downstream forward: _UP4_TAAACCTGCATGGCACACGT
  • References

    Reviews

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    Original publications

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