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mtlR [2019-07-23 09:16:21]
Molecular weight
78.67 kDa
Function
regulation of mannitol utilization
Product
transcriptional activator, PRD-type
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
467,130 469,214
The protein
Protein family
Domains
N-terminal DNA-binding domains, two PTS-regulation domains (PRD1 and PRD2), EIIB (Gat)-like domain, EIIA (Mtl)-like domain Modification
Effectors of protein activity
activity is stimulated by PtsH-dependent phosphorylation in PRD2 (mechnism of carbon catabolite repression) PubMedactivity is inhibited by MtlF-dependent phosphorylation in the EIIB(Gat)-like domain on Cys-419 (this prevents activity in the absence of mannitol and allows induction in presence of mannitol) PubMed Localization
upon interaction with non-phosphorylated MtlA, the active MtlR localizes to the membrane PubMed Expression and Regulation
Biological materials
Mutant
BKE04160 (mtlR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAAAAAGACCTCCTAGC, downstream forward: _UP4_TAAACCTGCATGGCACACGTBKK04160 (mtlR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAAAAAGACCTCCTAGC, downstream forward: _UP4_TAAACCTGCATGGCACACGT References
Reviews
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Original publications
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