transcriptional activator of the mtlA-mtlF-mtlD operon
function
regulation of mannitol utilization
product
transcriptional activator, PRD-type
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.10|Utilization of mannitol][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.3|PRD-type regulators][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.3|Phosphorylation on a Cys residue][SW|Categories] containing this gene/protein
[SW|utilization of specific carbon sources], [SW|transcription factors and their control], [SW|phosphoproteins]This gene is a member of the following [SW|regulons]
[SW|CcpA regulon]The protein
[SW|Domains]
N-terminal DNA-binding domains, two [SW|PTS]-regulation domains (PRD1 and PRD2), EIIB (Gat)-like domain, EIIA (Mtl)-like domainModification
phosphorylated on His-342 and/or His-399 by [protein|search|PtsH] [Pubmed|20444094]phosphorylation on His-599 by [protein|search|MtlA] [Pubmed|20444094]phosphorylation on Cys-419 by [protein|search|MtlF] [Pubmed|20444094]Effectors of protein activity
activity is stimulated by [protein|search|PtsH]-dependent phosphorylation in PRD2 (mechnism of carbon catabolite repression) [Pubmed|20444094]activity is inhibited by [protein|search|MtlF]-dependent phosphorylation in the EIIB(Gat)-like domain on Cys-419 (this prevents activity in the absence of mannitol and allows induction in presence of mannitol) [Pubmed|20444094][SW|Localization]
upon interaction with non-phosphorylated [protein|search|MtlA], the active [protein|search|MtlR] localizes to the membrane [Pubmed|23279188][SW|Interactions]
[protein|search|PtsH]-[protein|search|MtlR] [Pubmed|20444094][protein|search|MtlA]-[protein|search|MtlR] [Pubmed|20444094] (the non-phosphorylated IIB domain of [protein|search|MtlA] interacts with the two C-terminal EIIB (Gat)-like and EIIA (Mtl)-like domains of [protein|search|MtlR]) [Pubmed|23279188][protein|search|MtlF]-[protein|search|MtlR] [Pubmed|20444094]Expression and Regulation
Operon
''mtlR'' [Pubmed|12897001][SW|Sigma factor]
[protein|search|SigA] [Pubmed|22014119]Regulation
repressed by glucose ([protein|search|CcpA]) [Pubmed|22900538]Regulatory mechanism
[protein|search|CcpA]: transcription repression [Pubmed|22900538]References
Reviews
Original publications
12897001,20444094,10627040,10702268,9988713,23279188,22900538,22014119,26159071 
