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mtlR [2019-06-25 11:22:27]
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mtlR [2019-06-25 11:22:27]

transcriptional activator of the mtlA-mtlF-mtlD operon
Locus
BSU04160
Isoelectric point
5.31
Molecular weight
78.67 kDa
Protein length
694 aa Sequence Blast
Gene length
2085 bp Sequence Blast
Function
regulation of mannitol utilization
Product
transcriptional activator, PRD-type
Essential
no
Synonyms
ydaA

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
467,130 469,214

The protein

Protein family

Domains

  • N-terminal DNA-binding domains, two PTS-regulation domains (PRD1 and PRD2), EIIB (Gat)-like domain, EIIA (Mtl)-like domain
  • Modification

  • phosphorylated on His-342 and/or His-399 by PtsH PubMed
  • phosphorylation on His-599 by MtlA PubMed
  • phosphorylation on Cys-419 by MtlF PubMed
  • Effectors of protein activity

  • activity is stimulated by PtsH-dependent phosphorylation in PRD2 (mechnism of carbon catabolite repression) PubMed
  • activity is inhibited by MtlF-dependent phosphorylation in the EIIB(Gat)-like domain on Cys-419 (this prevents activity in the absence of mannitol and allows induction in presence of mannitol) PubMed
  • Localization

  • upon interaction with non-phosphorylated MtlA, the active MtlR localizes to the membrane PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • Regulation

  • repressed by glucose (CcpA) PubMed
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    Biological materials

    Mutant

  • BKE04160 (mtlR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAAAAAGACCTCCTAGC, downstream forward: _UP4_TAAACCTGCATGGCACACGT
  • BKK04160 (mtlR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAAAAAGACCTCCTAGC, downstream forward: _UP4_TAAACCTGCATGGCACACGT
  • References

    Reviews

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    Original publications

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