mtlR

transcriptional activator of the [gene|5291FEF41CA547223EDF9EBAFA38D3EE550ED3F3|mtlA]-[gene|EE4A4336E25F5DCAC89214F55CDE203AB47AA249|mtlF]-[gene|4D54898D405EA25A0C74B1F02A492143D49B7B07|mtlD] operon

locus: BSU_04160

pI: 5.31

mw: 78.67 kDa

protein length: 694 aa

gene length: 2085 bp

function: regulation of mannitol utilization

product: transcriptional activator, PRD-type

essential: no

synonyms: ydaA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.10|Utilization of mannitol]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.3|PRD-type regulators]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.3|Phosphorylation on a Cys residue]

Gene

Coordinates: 467,130 469,214

The protein

Catalyzed reaction/ biological activity

• D-mannitol + N^π-phospho-L-histidyl-[protein] --> D-mannitol 1-phosphate + L-histidyl-[protein] (according to UniProt)

Protein family

• [SW|PRD-containing transcription factors]

[SW|Domains]

• N-terminal DNA-binding domains, two [SW|PTS]-regulation domains (PRD1 aa 195-300 and PRD2 aa 305-410), EIIB (Gat)-like domain, EIIA (Mtl)-like domain
• [SW|PTS EIIB domain] type-2 (aa 413-502) (according to UniProt)
• [SW|PTS EIIA domain] type-2 (aa 536-683) (according to UniProt)

Modification

• phosphorylated on His-342 and/or His-399 by [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|PtsH] [Pubmed|20444094]
• phosphorylation on His-599 by [protein|5291FEF41CA547223EDF9EBAFA38D3EE550ED3F3|MtlA] [Pubmed|20444094]
• phosphorylation on Cys-419 by [protein|EE4A4336E25F5DCAC89214F55CDE203AB47AA249|MtlF] [Pubmed|20444094]

Effectors of protein activity

• activity is stimulated by [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|PtsH]-dependent phosphorylation in PRD2 (mechnism of carbon catabolite repression) [Pubmed|20444094]
• activity is inhibited by [protein|EE4A4336E25F5DCAC89214F55CDE203AB47AA249|MtlF]-dependent phosphorylation in the EIIB(Gat)-like domain on Cys-419 (this prevents activity in the absence of mannitol and allows induction in presence of mannitol) [Pubmed|20444094]

[SW|Localization]

• upon interaction with non-phosphorylated [protein|5291FEF41CA547223EDF9EBAFA38D3EE550ED3F3|MtlA], the active [protein|1CEDC98C9EDD8F3DEA0AF4B2104DA1BF86E688C2|MtlR] localizes to the membrane [Pubmed|23279188]

Expression and Regulation

Operons

• genes: [gene|1CEDC98C9EDD8F3DEA0AF4B2104DA1BF86E688C2|mtlR]
  description: [Pubmed|12897001]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|22014119], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|22900538], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • repressed by glucose ([protein|search|CcpA]) [Pubmed|22900538]

Biological materials

Mutant

• BKE04160 ([gene|1CEDC98C9EDD8F3DEA0AF4B2104DA1BF86E688C2|mtlR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAAAGACCTCCTAGC, downstream forward: _UP4_TAAACCTGCATGGCACACGT
• BKK04160 ([gene|1CEDC98C9EDD8F3DEA0AF4B2104DA1BF86E688C2|mtlR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAAAGACCTCCTAGC, downstream forward: _UP4_TAAACCTGCATGGCACACGT

References

Reviews

• 23318733,9663674

Original publications

• 12897001,20444094,10627040,10702268,9988713,23279188,22900538,22014119,26159071