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ssbB [2018-02-10T15:35:46.000Z]
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ssbB [2018-02-10T15:35:46.000Z]

single-strand DNA-binding proteinrequired to internalize and to recombine ssDNA with homologous resident duplex
Locus
BSU36310
Isoelectric point
6.88
Molecular weight
12.35 kDa
Protein length
113 aa Sequence Blast
Gene length
339 bp Sequence Blast
Function
required for efficient genetic transformation
Product
single-strand DNA-binding protein
Essential
no
Synonyms
ywpH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Genetics → DNA replication
  • Information processing → Genetics → DNA repair/ recombination
  • Information processing → Genetics → DNA repair/ recombination → Other proteins
  • Information processing → Genetics → Genetic competence
  • Lifestyles → Exponential and early post-exponential lifestyles → Genetic competence
  • Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue
  • Groups of genes → Phosphoproteins → Phosphorylation on a Tyr residue

    • Gene

    Coordinates
    3,740,206 → 3,740,547

    The protein

    Catalyzed reaction/ biological activity

  • binds and protects internalized foreign ssDNA PubMed
  • RecA-ATP in concert with DprA and SsbA catalyzes DNA strand exchange, with SsbB as an accessory factor PubMed

    • Paralogous protein(s)

  • SsbA

    • Modification

  • phosphorylated on Arg-55 PubMed
  • phosphorylated on Tyr by PtkA, dephosphorylated by PtpZ PubMed

    • Effectors of protein activity

  • phosphorylation strongly increases DNA-binding activity PubMed

    • Structure

  • 3VDY (in complex with ssDNA) PubMed

    • Localization

  • localizes to cell poles in competent cells PubMed
  • localizes to the DNA entry pole during transformation PubMed
  • transiently localizes to the cell pole, and co-localizes with the DNA uptake machinery PubMed

    • Expression and Regulation

    Operons

    Genes
    ssbB
    Description
    PubMed

    Regulatory mechanism

  • ComK: activation, PubMed, in ComK regulon

    • Regulation

  • expressed at the onset of stationary phase PubMed
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    Genes
    ywpG-ssbB
    Description
    PubMed
    view in new tab

    Biological materials

    Mutant

  • MGNA-A591 (ywpH::erm), available at the NBRP B. subtilis, Japan
  • BKE36310 (ΔssbB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCGATTCCTCCTCTCCT, downstream forward: _UP4_TGACACCCCTGCTCCTCCCG
  • BKK36310 (ΔssbB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCGATTCCTCCTCTCCT, downstream forward: _UP4_TGACACCCCTGCTCCTCCCG

    • Expression vector

  • for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

    • References

    Reviews

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    Original publications

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