gntR

transcriptional repressor (GntR family) of the gntR-gntK-gntP-gntZ operon

locus: BSU_40050

pI: 5.26

mw: 28.13 kDa

protein length: 243 aa

gene length: 732 bp

function: regulation of gluconate utilization

product: transcriptional repressor of the gluconate operon

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.13|Utilization of gluconate]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 4,113,417 4,114,148

The protein

Protein family

• [SW|GntR family] of transcription factors

[SW|Domains]

• [SW|HTH gntR-type domain] (aa 17-83) (according to UniProt)

Effectors of protein activity

• gluconate acts as molecular inducer

Expression and Regulation

Operons

• genes: [gene|1C566E54F8EF1A17A365FC49DFDA452AC5D0CA64|gntR]-[gene|72404FA6ECD62AEA3EFDB934DFB8DA581875605C|gntK]-[gene|C6810A4ACD51EB8D14E5B30163348058196266A1|gntP]-[gene|7E7187AA2DC172018A63A7B9CE48124F30311639|gntZ]
  description: [pubmed|10746760,3020045]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3020045], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|1C566E54F8EF1A17A365FC49DFDA452AC5D0CA64|GntR]: repression, [Pubmed|3020045], in [regulon|1C566E54F8EF1A17A365FC49DFDA452AC5D0CA64|GntR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|10666464], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: activation, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • induced by gluconate ([protein|search|GntR]) [Pubmed|3020045]

Biological materials

Mutant

• BKE40050 ([gene|1C566E54F8EF1A17A365FC49DFDA452AC5D0CA64|gntR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACACTCACCTTCCTCAC, downstream forward: _UP4_CTGGCAAAAGGAGCTGAATA
• BKK40050 ([gene|1C566E54F8EF1A17A365FC49DFDA452AC5D0CA64|gntR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACACTCACCTTCCTCAC, downstream forward: _UP4_CTGGCAAAAGGAGCTGAATA

References

• 8370661,8808939,8288545,7642511,7476858,8596444,3011959,2163901,3037520,2492998,9106211,2419835,2124676,3020045,10746760,8510140,2843515,7476858,3020045,1659648,21106498,22900538,220817675,27829583