gmk

guanylate kinase (GMP:dATP, dGMP:ATP)

locus: BSU_15680

pI: 4.62

mw: 23.07 kDa

protein length: 204 aa

gene length: 615 bp

function: GTP biosynthesis

product: guanylate kinase (GMP:dATP, dGMP:ATP)

essential: yes

ec: 2.7.4.8

synonyms: yloD

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides]
• [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.3|Targets of (p)ppGpp]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

Gene

Coordinates: 1,641,949 1,642,563

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP + GMP --> ADP + GDP (according to UniProt)

Protein family

• guanylate kinase family (single member, according to UniProt)

[SW|Domains]

• Guanylate kinase-like domain (aa 5-184) (according to UniProt)

Modification

• phosphorylated on Arg-134 and/or Arg-149[Pubmed|22517742]

Effectors of protein activity

• inhibition of enzymatic activity by (p)ppGpp during the stringent response[Pubmed|22981860]

Structure

• [PDB|1S4Q] (from ''Mycobacterium tuberculosis h37rv'', 39% identity, 60% similarity)
• [http://pdb.org/pdb/search/structidSearch.do?structureId=4QRH 4QRH] (from ''Staphylococcus aureus'', complex with pppGpp) [Pubmed|25661490]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]

  description

  • [pubmed|22383849]

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|23396918], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulation

  • expressed during vegetative growth and early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|23396918]

• genes: [gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]-[gene|2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C|yloI]-[gene|AF5ADDA2A98EC18BB72A81AA0336E639C0D1F430|priA]-[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]

  description

  • [pubmed|22383849]

• genes: [gene|7821A09DADC6EAF589A882F05CC34BE900E06F2A|yloB]-[gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]

  description

  • [pubmed|22383849]

  regulation

  • expressed during sporulation in the mother cell [pubmed|12161109]

References

Reviews

• 27149325

Original publications

• 22517742,22981860,24722991,24682323,25661490,31552824