trxB

thioredoxin reductase (NADPH)

locus: BSU_34790

pI: 4.99

mw: 34.36 kDa

protein length: 316 aa

gene length: 951 bp

function: keeps [protein|4E5C84FDC8FE2FEFF47306C91ECAB7F17D3E38E9|thioredoxin] in the reduced state

product: thioredoxin reductase (NADPH)

essential: yes

ec: 1.8.1.9

synonyms: yvcH,trxR

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.3|Electron transport/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 3,573,207 3,574,157

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• [protein|4E5C84FDC8FE2FEFF47306C91ECAB7F17D3E38E9|Thioredoxin] + NADP⁺ --> [protein|4E5C84FDC8FE2FEFF47306C91ECAB7F17D3E38E9|thioredoxin] disulfide + NADPH [pubmed|30855939]

Protein family

• class-II pyridine nucleotide-disulfide oxidoreductase family (with [protein|8F23B575BC32EA63267C4321DD8E4BD546B11AF1|AhpF], according to UniProt)

[SW|Cofactors]

• FAD [Pubmed|30855939]

Structure

• [PDB|4GCM] (from ''S. aureus, 71% identity'')

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|1BC439BBCD5FE19D5780519D7E4317C2EFF0D21B|trxB]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|29271514,12642660], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

  regulation

  • induced by cell wall stress ([protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]) [pubmed|29271514]

References

Reviews

• 23899494

Original publications

• 20084284,12642660,20525796,24401092,29271514,30855939