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ywlE [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]
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ywlE [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]

protein arginine phosphatase
locus
BSU36930
pI
6.00
mw
16.00 kDa
function
dephosphorylation of McsB
product
protein arginine phosphatase
essential
no
ec
3.1.3.48
synonyms
ipc-31d
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.4|Protein phosphatases]

    • [SW|Categories] containing this gene/protein

  • [SW|protein modification]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,791,805 -> 3,792,257

    The protein

    Catalyzed reaction/ biological activity

  • Protein arginine phosphate H2O = protein arginine phosphate [Pubmed|22517742]

    • Paralogous protein(s)

  • [protein|search|YfkJ]

    • Effectors of protein activity

  • activity is inhibited by oxidative stress [Pubmed|27524296]

    • Structure

  • [PDB|1ZGG]

    • [SW|Localization]

  • weak oligomerization, results in inactivation of YwlE [Pubmed|19678837]

    • [SW|Interactions]

  • [protein|search|McsB]-[protein|search|YwlE] (dephosphorylation of [protein|search|McsB]) [Pubmed|20852588,21622759]

    • Biological materials

    Mutant

  • ''ywlE::aphA3'' available from [SW|Ulf Gerth]'s lab
  • ''ywlE::tet'' available from [SW|Ulf Gerth]'s lab
  • ''ywlE::spec'' available from [SW|Ulf Gerth]'s lab
  • GP1458 (''ywlE''::''aphA3''), available in [SW|Jörg Stülke]'s lab
  • GP1459 (''ywlE''::''tet''), available in [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs [Pubmed|25610436]

    • Expression vector

  • for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [SW|Ulf Gerth]'s lab
  • expression of native ''ywlE'' in ''B. subtilis'': pBP183 (in [SW|pBQ200]), available in [SW|Fabian Commichau]'s lab

    • Antibody

  • available in [SW|Ulf Gerth]'s lab

    • Labs working on this gene/protein

  • [SW|Ulf Gerth], Greifswald, Germany
  • [SW|Fabian Commichau] Göttingen, Germany

    • References

    Reviews

  • 27541193

    • Original Publications

  • 16452434,15866923,15995210,19678837,21622759,20852588,22517742,23770242,23838530,24263382,24825175,25610436, 27524296