SubtiBank SubtiBank
ywlE
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ywlE

protein arginine phosphatase
Locus
BSU_36930
Isoelectric point
6.68
Molecular weight
16.64 kDa
Protein length
150 aa Sequence Blast
Gene length
453 bp Sequence Blast
Function
dephosphorylation of McsB
Product
protein arginine phosphatase
Essential
no
E.C.
3.1.3.48
Synonyms
ipc-31d
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Protein modification → Protein phosphatases

    • Gene

    Coordinates
    3,791,805 3,792,257

    Phenotypes of a mutant

  • impaired spore germination PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • Protein arginine phosphate + H2O --> protein arginine + phosphate PubMed

    • Protein family

  • low molecular weight phosphotyrosine protein phosphatase family (with ArsC and YfkJ, according to UniProt)

    • Paralogous protein(s)

  • YfkJ

    • Effectors of protein activity

  • activity is inhibited by oxidative stress PubMed

    • Structure

  • 1ZGG

    • Localization

  • weak oligomerization, results in inactivation of YwlE PubMed

    • Expression and Regulation

    Operons

    Genes
    ywlD-ywlE-ywlF-ywlG
    Description
    PubMed
    view in new tab

    Genes
    ywlE-ywlF-ywlG
    Description
    PubMed
    view in new tab

    Biological materials

    Mutant

  • MGNA-A186 (ywlE::erm), available at the NBRP B. subtilis, Japan
  • ywlE::aphA3 available from Ulf Gerth's lab
  • ywlE::tet available from Ulf Gerth's lab
  • ywlE::spec available from Ulf Gerth's lab
  • GP1458 (ywlE::aphA3), available in Jörg Stülke's lab
  • GP1459 (ywlE::tet), available in Jörg Stülke's and Fabian Commichau's labs PubMed
  • BKE36930 (ywlE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCAGTCACCCCTTATT, downstream forward: _UP4_TAAGTTGTCAGAAAATCTGC
  • BKK36930 (ywlE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCAGTCACCCCTTATT, downstream forward: _UP4_TAAGTTGTCAGAAAATCTGC

    • Expression vectors

  • for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Ulf Gerth's lab
  • expression of native ywlE in B. subtilis: pBP183 (in pBQ200), available in Fabian Commichau's lab

    • Antibody

  • available in Ulf Gerth's lab

    • Labs working on this gene/protein

  • Ulf Gerth, Greifswald, Germany
  • Fabian Commichau Göttingen, Germany homepage

    • References

    Reviews

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    Original Publications

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