ispF [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]

2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase, 5th step in the MEP pathway of isoprenoid biosynthesis

Locus: BSU00910

Isoelectric point: 5.40

Molecular weight: 16.00 kDa

Function: MEP pathway of isoprenoid biosynthesis

Product: 2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase

Essential: yes

E.C.: 4.6.1.12

Synonyms: yacN

Genomic Context

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Categories containing this gene/protein

• Metabolism → Lipid metabolism → Biosynthesis of lipids → Biosynthesis of isoprenoids
• Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
• Groups of genes → Essential genes

Categories containing this gene/protein

• biosynthesis of lipids, general stress proteins (controlled by SigB), essential genes

This gene is a member of the following regulons

• SigB regulon

Gene

Coordinates on the chromosome (coding sequence): 110,480 -> 110,956

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP (according to Swiss-Prot)

Protein family

• ispF family (according to Swiss-Prot)

Structure

• 1T0A (from Shewanella oneidensis, 62% identity) PubMed

Expression and Regulation

Operon

• yacL-ispD-ispF PubMed

Sigma factor

• SigB PubMed

Regulation

• induced by stress (SigB) PubMed

References

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