ispF [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]

2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase, 5th step in the MEP pathway of isoprenoid biosynthesis

locus: BSU00910

pI: 5.40

mw: 16.00 kDa

function: MEP pathway of isoprenoid biosynthesis

product: 2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase

essential: yes

ec: 4.6.1.12

synonyms: yacN

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

[SW|Categories] containing this gene/protein

• [SW|biosynthesis of lipids], [SW|general stress proteins (controlled by SigB)], [SW|essential genes]

This gene is a member of the following [SW|regulons]

• [SW|SigB regulon]

Gene

Coordinates on the chromosome (coding sequence): 110,480 -> 110,956

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP (according to Swiss-Prot)

Protein family

• ispF family (according to Swiss-Prot)

Structure

• [PDB|1T0A] (from ''Shewanella oneidensis'', 62% identity) [Pubmed|15502301]

Expression and Regulation

Operon

• ''[protein|search|yacL]-[protein|search|ispD]-[protein|search|ispF]'' [Pubmed|10482513]

[SW|Sigma factor]

• [protein|search|SigB] [Pubmed|15805528]

Regulation

• induced by stress ([protein|search|SigB]) [Pubmed|15805528]

References

• 15805528,10482513,12270818,17458547,23840410,15502301