ispF [2019-07-19 10:17:04]

2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase, 5th step in the MEP pathway of isoprenoid biosynthesis

Locus: BSU_00910

Isoelectric point: 5.40

Molecular weight: 16.98 kDa

Protein length: 158 aa

Gene length: 477 bp

Function: MEP pathway of isoprenoid biosynthesis

Product: 2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase

Essential: yes

E.C.: 4.6.1.12

Synonyms: yacN

Genomic Context

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Categories containing this gene/protein

• Metabolism → Lipid metabolism → Biosynthesis of lipids → Biosynthesis of isoprenoids
• Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
• Groups of genes → Essential genes

Gene

Coordinates: 110,480 110,956

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP (according to Swiss-Prot)

Protein family

• IspF family (single member, according to UniProt)

Structure

• 5IWX (native) PubMed
• 5IWY (complex with CMP) PubMed

Expression and Regulation

Operons

• Genes: yacL-ispD-ispF
  Description: PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon
  • SigM: sigma factor, PubMed, in SigM regulon

  Regulation

  • induced by stress (SigB) PubMed

Biological materials

Mutant

• MGNA-B882 (yacN::erm), available at the NBRP B. subtilis, Japan

References

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