ycbG

transcription regulator of the glucarate/galactarate utilization operon (GntR family)

Locus: BSU_02500

Isoelectric point: 5.99

Molecular weight: 26.43 kDa

Protein length: 233 aa

Gene length: 702 bp

Function: regulation of glucarate/galactarate utilization

Product: transcription regulator (GntR family)

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of glucarate/galactarate
• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other

Gene

Coordinates: 273,237 273,938

The protein

Protein family

• GntR family of transcription factors

Domains

• HTH gntR-type domain (aa 16–84) (according to UniProt)

Structure

• 2DI3 (from Corynebacterium glutamicum, corresponds to aa 20 ... 178, 32.7% identity) PubMed

Expression and Regulation

Operons

• Genes: ycbC-ycbD-ycbE-ycbF-ycbG-ycbH-ycbJ
  Description: PubMed

  Regulatory mechanism

  • YcbG: repression, PubMed, in YcbG regulon
  • AbrB: repression, PubMed, in AbrB regulon

  Regulation

  • induced by D-glucarate/galactarate (YcbG) PubMed

Biological materials

Mutant

• MGNA-C030 (ycbG::erm), available at the NBRP B. subtilis, Japan
• BKE02500 (ycbG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGAACAACTCTCCCTTCA, downstream forward: _UP4_TAATGGTGAGCAACCGCAGT
• BKK02500 (ycbG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGAACAACTCTCCCTTCA, downstream forward: _UP4_TAATGGTGAGCAACCGCAGT

References

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