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ddcP
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ddcP

DNA damage checkpoint recovery protease, promotes DNA damage checkpoint recovery
locus
BSU_15050
pI
5.85
mw
38.61 kDa
protein length
341 aa Sequence Blast
gene length
1026 bp Sequence Blast
function
degradation of [protein|7BF591DCDC9635C605D76135481A8A9DB63EE861|YneA]
product
DNA damage checkpoint recovery protease
essential
no
synonyms
ylbL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,572,765 1,573,790

    Phenotypes of a mutant

  • deletion of [protein|5ED8301B9681FDD5171A9344A971E891493A6558|CtpA] and [protein|1A5C1BDE844AA56B90C0E4A02D74978676487E99|DdcP] leads to accumulation of the checkpoint protein [protein|7BF591DCDC9635C605D76135481A8A9DB63EE861|YneA] [pubmed|29979679]
  • sensitivity to DNA damage [pubmed|29979679]

    • The protein

    Protein family

  • Peptidase S16 family (with [protein|FF15BE26BCC78EC1301C58A51AF0A519D7BE9ADC|LonA] and [protein|CA90AAE5320C20F93F5ED0F167294C28495756FF|LonB], according to UniProt)

    • [SW|Domains]

  • TM helix (aa 9 - 29) [pubmed|29979679]
  • [SW|PDZ domain] (aa 100 - 186) [pubmed|29979679]
  • Lon protease domain (aa 187 - 336) [pubmed|29979679]

    • Structure

  • [PDB|2KJP] (PDZ domain)

    • [SW|Localization]

  • cell membrane, with extracellular protease domain [pubmed|30315724]

    • Expression and Regulation

    Operons

    genes
    [gene|DE3BCF20DE5BB438ACBB36B412817F6124CFDFFC|ylbK]-[gene|1A5C1BDE844AA56B90C0E4A02D74978676487E99|ddcP]
    description
    [pubmed|22383849]
    view in new tab

    genes
    [gene|1A5C1BDE844AA56B90C0E4A02D74978676487E99|ddcP]
    description
    [pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-B249 (ylbL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1248 NBRP B. subtilis, Japan]
  • BKE15050 ([gene|1A5C1BDE844AA56B90C0E4A02D74978676487E99|ddcP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCTAAAATGTTTTTTACGTA, downstream forward: _UP4_CTGAAAGCGAAAAGCACCTG
  • BKK15050 ([gene|1A5C1BDE844AA56B90C0E4A02D74978676487E99|ddcP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCTAAAATGTTTTTTACGTA, downstream forward: _UP4_CTGAAAGCGAAAAGCACCTG

    • References

    Research papers

  • 29979679,30315724