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yxeK [2018-04-08 17:48:51]
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yxeK [2018-04-08 17:48:51]

N-acetyl S-(2-succino)cysteine oxygenase
Locus
BSU39520
Isoelectric point
5.67
Molecular weight
49.18 kDa
Protein length
441 aa Sequence Blast
Gene length
1323 bp Sequence Blast
Function
utilization of S-(2-succino)cysteine
Product
N-acetyl S-(2-succino)cysteine oxygenase
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
4,060,818 → 4,062,143

Phenotypes of a mutant

  • no growth with S-(2-succino)cysteine as the single source of sulfur PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • oxygenation of N-acetyl S-(2-succino)cysteine PubMed
  • Protein family

  • ntaA/snaA/soxA(dszA) monooxygenase family (according to Swiss-Prot)
  • Paralogous protein(s)

    Structure

  • 5TLC (from B. subtilis WU-S2B 42% identity) PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • CymR: repression, PubMed, in CymR regulon
  • Regulation

  • repressed in the presence of cysteine (CymR) PubMed
  • Additional information

  • the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed
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    Biological materials

    Mutant

  • MGNA-B709 (yxeK::erm), available at the NBRP B. subtilis, Japan
  • BKE39520 (ΔyxeK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGCGATACTCCTTTTAT, downstream forward: _UP4_TATGCAAAGTAAGGAGGAAC
  • BKK39520 (ΔyxeK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGCGATACTCCTTTTAT, downstream forward: _UP4_TATGCAAAGTAAGGAGGAAC
  • References

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