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lipM
function
biosynthesis of lipoic acid
product
octanoyltransferase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.7|Biosynthesis and scavenging of lipoic acid]Gene
Coordinates
2,543,968 2,544,804
The protein
Catalyzed reaction/ biological activity
transfers the octanoyl moiety from octanoyl-acyl carrier protein to the lipoyl domains of the 2-oxoacid dehydrogenases via a thioester-linked octanoyl-LipM intermediate [Pubmed|20882995]L-lysyl-[protein] + octanoyl-[ACP] --> H+ + holo-[ACP] + N6-octanoyl-L-lysyl-[protein] (according to UniProt)Protein family
octanoyltransferase LipM family (single member, according to UniProt)[SW|Domains]
[SW|BPL/LPL catalytic domain] (aa 33-248) (according to UniProt)Structure
[PDB|3A7A] (from ''E. coli'', 25% identity) [Pubmed|20089862][SW|Localization]
cytoplasm (according to Swiss-Prot)Expression and Regulation
Operons
genes
[gene|19CEB80CF637F1FED7C493EF743B4BFA632C8D44|lipM]-[gene|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR]
description
[pubmed|22383849]
view in new tabBiological materials
Mutant
MGNA-C469 (yqhM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2467 NBRP B. subtilis, Japan]BKE24530 ([gene|19CEB80CF637F1FED7C493EF743B4BFA632C8D44|lipM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTAAGACCTTCCTTAC, downstream forward: _UP4_TAAACAGGGCTTTTGACCCGBKK24530 ([gene|19CEB80CF637F1FED7C493EF743B4BFA632C8D44|lipM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTAAGACCTTCCTTAC, downstream forward: _UP4_TAAACAGGGCTTTTGACCCGReferences
Reviews
Original publications