lipM

octanoyltransferase

Locus: BSU_24530

Isoelectric point: 5.93

Molecular weight: 32.09 kDa

Protein length: 278 aa

Gene length: 837 bp

Function: biosynthesis of lipoic acid

Product: octanoyltransferase

Essential: no

E.C.: 2.3.1.181

Synonyms: yqhM

Genomic Context

Loading

Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis and scavenging of lipoic acid

Gene

Coordinates: 2,543,968 2,544,804

The protein

Catalyzed reaction/ biological activity

• transfers the octanoyl moiety from octanoyl-acyl carrier protein to the lipoyl domains of the 2-oxoacid dehydrogenases via a thioester-linked octanoyl-LipM intermediate PubMed
• L-lysyl-[protein] + octanoyl-[ACP] --> H⁺ + holo-[ACP] + N⁶-octanoyl-L-lysyl-[protein] (according to UniProt)

Protein family

• octanoyltransferase LipM family (single member, according to UniProt)

Domains

• BPL/LPL catalytic domain (aa 33-248) (according to UniProt)

Structure

• 3A7A (from E. coli, 25% identity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: lipM-mntR
  Description: PubMed

Biological materials

Mutant

• MGNA-C469 (yqhM::erm), available at the NBRP B. subtilis, Japan
• BKE24530 (lipM::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTAAGACCTTCCTTAC, downstream forward: _UP4_TAAACAGGGCTTTTGACCCG
• BKK24530 (lipM::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTAAGACCTTCCTTAC, downstream forward: _UP4_TAAACAGGGCTTTTGACCCG

References

Reviews

Loading

Original publications

Loading