lipM

octanoyltransferase

locus: BSU_24530

pI: 5.93

mw: 32.09 kDa

protein length: 278 aa

gene length: 837 bp

function: biosynthesis of lipoic acid

product: octanoyltransferase

essential: no

ec: 2.3.1.181

synonyms: yqhM

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.7|Biosynthesis and scavenging of lipoic acid]

Gene

Coordinates: 2,543,968 2,544,804

The protein

Catalyzed reaction/ biological activity

• transfers the octanoyl moiety from octanoyl-acyl carrier protein to the lipoyl domains of the 2-oxoacid dehydrogenases via a thioester-linked octanoyl-LipM intermediate [Pubmed|20882995]
• L-lysyl-[protein] + octanoyl-[ACP] --> H⁺ + holo-[ACP] + N⁶-octanoyl-L-lysyl-[protein] (according to UniProt)

Protein family

• octanoyltransferase LipM family (single member, according to UniProt)

[SW|Domains]

• [SW|BPL/LPL catalytic domain] (aa 33-248) (according to UniProt)

Structure

• [PDB|3A7A] (from ''E. coli'', 25% identity) [Pubmed|20089862]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|19CEB80CF637F1FED7C493EF743B4BFA632C8D44|lipM]-[gene|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C469 (yqhM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2467 NBRP B. subtilis, Japan]
• BKE24530 ([gene|19CEB80CF637F1FED7C493EF743B4BFA632C8D44|lipM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTAAGACCTTCCTTAC, downstream forward: _UP4_TAAACAGGGCTTTTGACCCG
• BKK24530 ([gene|19CEB80CF637F1FED7C493EF743B4BFA632C8D44|lipM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTAAGACCTTCCTTAC, downstream forward: _UP4_TAAACAGGGCTTTTGACCCG

References

Reviews

• 27074917

Original publications

• 20882995,20089862