albC [2019-09-02 12:52:53]

[SW|ABC transporter ](ATP-binding protein), export of antilisterial bacteriocin (subtilosin)

locus: BSU_37390

pI: 5.25

mw: 27.08 kDa

protein length: 239 aa

gene length: 720 bp

function: export of antilisterial bacteriocin (subtilosin)

product: [SW|ABC transporter ](ATP-binding protein)

essential: no

synonyms: ywhQ

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.2|Export of antibiotic substances]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,837,840 3,838,559

The protein

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

Structure

• [PDB|4RVC] (from Geobacillus kaustophilus, 30% identity) [pubmed|25724946]

[SW|Localization]

• membrane associated (via [protein|DAD8308DF72E648610D1A12BC92063E1550CB1B4|AlbD]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|8CBBEC47A5CC3EAE73BE68E68417C5F2A4DA5C23|sboA]-[gene|83AAFAA187B4FCEE24B4EA2DD4935425A3B48DAA|sboX]-[gene|DEDA6F03386F360F6C0B2CC5F09DF3C9D85173F1|albA]-[gene|D399B25AE22ECB6C7ACDCFF4DCE4D9D908184E75|albB]-[gene|19CC58F5ABADAD45BA55D88BBD77B586AA5D85FE|albC]-[gene|DAD8308DF72E648610D1A12BC92063E1550CB1B4|albD]-[gene|A474F01AA4AD859963E39CD528BC42FF43A72433|albE]-[gene|D03FB4EAEFBC25A5B0459CF464BFADF1FC7620B2|albF]-[gene|55A34610EF7AA5E515DE3DCDA333ED3A42B0EF08|albG]
  description: [Pubmed|10572140]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10572140], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]: repression, [Pubmed|15743949], in [regulon|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok regulon]
  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: activation, [Pubmed|10809710], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [PubMed|10572140,17720793], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • expressed under anaerobic conditions ([protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]) [Pubmed|10809710]
  • expression of the operon is strongly induced at the beginning of [SW|biofilm formation] [pubmed|31113899]

Biological materials

Mutant

• MGNA-A869 (ywhQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/869 NBRP B. subtilis, Japan]
• BKE37390 ([gene|19CC58F5ABADAD45BA55D88BBD77B586AA5D85FE|albC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACATCGTGTATATCCAAAA, downstream forward: _UP4_CGGGAATTTTTCGAGGTGAT
• BKK37390 ([gene|19CC58F5ABADAD45BA55D88BBD77B586AA5D85FE|albC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACATCGTGTATATCCAAAA, downstream forward: _UP4_CGGGAATTTTTCGAGGTGAT

References

• 10809709,10092453,10572140,15743949,10809710,10572140,17720793,25724946