nupN

lipoprotein, part of guanosine transporter

Locus: BSU_31540

Isoelectric point: 4.82

Molecular weight: 37.19 kDa

Protein length: 359 aa

Gene length: 1080 bp

Function: uptake of guanosine

Product: lipoprotein, part of guanosine transporter

Essential: no

Synonyms: yufN

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of carbon sources
• Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of purine nucleotides
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,239,930 3,241,009

Phenotypes of a mutant

• a nupN nupG double mutant is unable to utilize externally provided guanosine PubMed

The protein

Protein family

• BMP lipoprotein family (with Med, according to UniProt)

Structure

• 2FQW (from Treponema pallidum, 45% identity) PubMed

Localization

• cell membrane (according to UniProt), membrane associated PubMed

Expression and Regulation

Operons

• Genes: nupN-nupO-nupP-nupQ
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CodY: repression, PubMed, in CodY regulon

  Regulation

  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

Biological materials

Mutant

• MGNA-B553 (yufN::erm), available at the NBRP B. subtilis, Japan
• BKE31540 (nupN::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGATATAACCCCCCAGAA, downstream forward: _UP4_TAAGAATCGCAGGGATAAGG
• BKK31540 (nupN::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGATATAACCCCCCAGAA, downstream forward: _UP4_TAAGAATCGCAGGGATAAGG

References

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