yvcL [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

WhiA family protein, involved in Z ring assembly, putative transcription factor

Locus: BSU34750

Isoelectric point: 9.48

Molecular weight: 36.00 kDa

Function: control of Z ring asembly

Product: cell division protein

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell division → Other genes
• Information processing → Genetics → DNA condensation/ segregation
• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factor/ other/ based on similarity

Categories containing this gene/protein

• cell division, transcription factors and their control

Gene

Coordinates on the chromosome (coding sequence): 3,569,573 -> 3,570,523

Phenotypes of a mutant

• a yvcL zapA double mutant grows poorly, and the cells are filamentous (due to a defect in Z ring assembly) PubMed
• a yvcL ezrA double mutant grows poorly, and the cells are filamentous PubMed
• a yvcL minC-minD mutant grows poorly, and the cells are filamentous PubMed
• a yvcL noc double mutant grows poorly, and the cells are filamentous PubMed
• the defects of the yvcL zapA double mutant can be suppressed by inactivation of either gtaB, pgcA, or ugtP PubMed

The protein

Protein family

• WhiA family PubMed

Domains

• suggested HTH domain at the N terminus PubMed

Structure

• 3HYI (Crystal structure of full-length DUF199/WhiA from Thermotoga maritima)
• 3HYJ (Crystal structure of the N-terminal LAGLIDADG domain of DUF199/WhiA)

Localization

• localizes to the nucleoid PubMed

Expression and Regulation

Operon

• yvcI-yvcJ-yvcK-yvcL-crh-yvcN PubMed

Sigma factor

• SigA PubMed

Regulation

• constitutive expression at both protein and RNA levels PubMed
• very weak stimuation of expression by citrate and succinate PubMed

Biological materials

Mutant

• GP231 (amyE::PrpsJ-lacZ, yvcL::cat) available in Jörg Stülke's lab; plasmid pGP1468 was used to disrupt the gene.

Expression vector

• pGP1469 for expression in B. subtilis (in pGP382, with C-terminal STREP-TAG), available in Jörg Stülke's lab
• pGP2429 (N-terminal Strep-Tag, purification from E. coli and/or B. subtilis, in pDG148), for SPINE, available in Jörg Stülke's lab
• pGP2430 (C-terminal Strep-Tag, purification from E. coli and/or B. subtilis, in pDG148), for SPINE, available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's and Boris Görke's labs

FLAG-tag construct

• GP782 (spc, based on pGP1331), available in Jörg Stülke's lab

Antibody

• available in Leendert Hamoen's lab PubMed

References

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