yvcL [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

WhiA family protein, involved in Z ring assembly, putative transcription factor

locus: BSU34750

pI: 9.48

mw: 36.00 kDa

function: control of Z ring asembly

product: cell division protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.6|Transcription factor/ other/ based on similarity]

[SW|Categories] containing this gene/protein

• [SW|cell division], [SW|transcription factors and their control]

Gene

Coordinates on the chromosome (coding sequence): 3,569,573 -> 3,570,523

Phenotypes of a mutant

• a ''[protein|search|yvcL] [protein|search|zapA]'' double mutant grows poorly, and the cells are filamentous (due to a defect in Z ring assembly) [Pubmed|24097947]
• a ''[protein|search|yvcL] [protein|search|ezrA]'' double mutant grows poorly, and the cells are filamentous [Pubmed|24097947]
• a ''[protein|search|yvcL] [protein|search|minC]-[protein|search|minD]'' mutant grows poorly, and the cells are filamentous [Pubmed|24097947]
• a ''[protein|search|yvcL] [protein|search|noc]'' double mutant grows poorly, and the cells are filamentous [Pubmed|24097947]
• the defects of the ''[protein|search|yvcL] [protein|search|zapA]'' double mutant can be suppressed by inactivation of either ''[protein|search|gtaB]'', ''[protein|search|pgcA]'', or ''[protein|search|ugtP]'' [Pubmed|24097947]

The protein

Protein family

• WhiA family [Pubmed|10986251]

[SW|Domains]

• suggested HTH domain at the N terminus [Pubmed|19836336]

Structure

• [PDB|3HYI] (Crystal structure of full-length DUF199/WhiA from ''Thermotoga maritima'')
• [PDB|3HYJ] (Crystal structure of the N-terminal LAGLIDADG domain of DUF199/WhiA)

[SW|Localization]

• localizes to the nucleoid [Pubmed|24097947]

Expression and Regulation

Operon

• ''[protein|search|yvcI]-[protein|search|yvcJ]-[protein|search|yvcK]-[protein|search|yvcL]-[protein|search|crh]-[protein|search|yvcN]'' [Pubmed|9237995]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|16272399]

Regulation

• constitutive expression at both protein and RNA levels [Pubmed|24097947,22383849]
• very weak stimuation of expression by citrate and succinate [Pubmed|16272399]

Biological materials

Mutant

• GP231 (''amyE::P[protein|search|rpsJ]-lacZ, yvcL::cat'') available in [SW| Jörg Stülke]'s lab; plasmid pGP1468 was used to disrupt the gene.

Expression vector

• pGP1469 for expression in ''B. subtilis'' (in [SW|pGP382], with C-terminal STREP-TAG), available in [SW| Jörg Stülke]'s lab
• pGP2429 (N-terminal Strep-Tag, purification from ''E. coli'' and/or ''B. subtilis'', in pDG148), for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
• pGP2430 (C-terminal Strep-Tag, purification from ''E. coli'' and/or ''B. subtilis'', in pDG148), for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW| Jörg Stülke]'s and [SW|Boris Görke]'s labs

FLAG-tag construct

• GP782 (''spc'', based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

Antibody

• available in [SW|Leendert Hamoen]'s lab [Pubmed|24097947]

References

• 9237995,16272399,10986251,19836336,17603302,24097947