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tyrZ [Mon Aug 31 2015 13:57:30 GMT+0200 (CEST)]
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tyrZ [Mon Aug 31 2015 13:57:30 GMT+0200 (CEST)]

alternative tyrosyl-tRNA synthetase, required to prevent misincorporation of D-Tyr into proteins
locus
BSU38460
pI
6.66
mw
46.00 kDa
function
translation
product
tyrosyl-tRNA synthetase (minor)
essential
no
ec
6.1.1.1
synonyms
ipa-9r, tyrS1, tyrT
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]
    • syns
    ipa-9r, tyrS1, tyrT

    [SW|Categories] containing this gene/protein

  • [SW|translation]

    • This gene is a member of the following [SW|regulons]

  • [SW|DtrR regulon], [SW|T-box]

    • Gene

    Phenotypes of a mutant

  • sensitive against D-Tyr [Pubmed|25733610]
  • a ''[protein|search|tyrS] [protein|search|tyrZ]'' double mutant is not viable [Pubmed|25733610]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot) ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot)
  • [protein|search|TyrZ] is highly specific for L-Tyr, prevents misincorporation of D-Tyr into proteins, less processive than [protein|search|TyrS] [Pubmed|25733610]

    • Protein family

  • TyrS type 2 subfamily (according to Swiss-Prot), class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)

    • Modification

  • phosphorylated on Thr-311 [Pubmed|20509597]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operon

  • ''[protein|search|dtrR]-[protein|search|tyrZ]'' [Pubmed|25733610]

    • [SW|Sigma factor]

  • ''[protein|search|tyrZ]'' [Pubmed|1735721]

    • Regulation

  • induced by tyrosine limitation ([protein|search|T-box]) [Pubmed|25733610,19258532,1735721]

    • Regulatory mechanism

  • [protein|search|T-box]: [SW|RNA switch], transcriptional antitermination [Pubmed|25733610,19258532,1735721]
  • [protein|search|DtrR]: transcriptional repression [Pubmed|25733610]

    • Biological materials

    Mutant

  • QB53532 (aphA3), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 19258532,10546897

    • Original publications

  • 1735721,25733610,20509597