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tyrZ [Mon Aug 31 2015 13:57:30 GMT+0200 (CEST)]
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tyrZ [Mon Aug 31 2015 13:57:30 GMT+0200 (CEST)]

alternative tyrosyl-tRNA synthetase, required to prevent misincorporation of D-Tyr into proteins
Locus
BSU38460
Isoelectric point
6.66
Molecular weight
46.00 kDa
Function
translation
Product
tyrosyl-tRNA synthetase (minor)
Essential
no
E.C.
6.1.1.1
Synonyms
ipa-9r, tyrS1, tyrT

Genomic Context

      
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Categories containing this gene/protein

Syns
ipa-9r, tyrS1, tyrT

Categories containing this gene/protein

This gene is a member of the following regulons

Gene

Phenotypes of a mutant

  • sensitive against D-Tyr PubMed
  • a tyrS tyrZ double mutant is not viable PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot) ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot)
  • TyrZ is highly specific for L-Tyr, prevents misincorporation of D-Tyr into proteins, less processive than TyrS PubMed
  • Protein family

  • TyrS type 2 subfamily (according to Swiss-Prot), class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Modification

  • phosphorylated on Thr-311 PubMed
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operon

    Sigma factor

    Regulation

  • induced by tyrosine limitation (T-box) PubMed
  • Regulatory mechanism

  • T-box: RNA switch, transcriptional antitermination PubMed
  • DtrR: transcriptional repression PubMed
  • Biological materials

    Mutant

  • QB53532 (aphA3), available in Jörg Stülke's lab
  • References

    Reviews

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    Original publications

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