tyrZ [Mon Aug 31 2015 13:57:30 GMT+0200 (CEST)]

alternative tyrosyl-tRNA synthetase, required to prevent misincorporation of D-Tyr into proteins

Locus: BSU38460

Isoelectric point: 6.66

Molecular weight: 46.00 kDa

Function: translation

Product: tyrosyl-tRNA synthetase (minor)

Essential: no

E.C.: 6.1.1.1

Synonyms: ipa-9r, tyrS1, tyrT

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Aminoacyl-tRNA synthetases
• Groups of genes → Phosphoproteins → Phosphorylation on a Thr residue

Syns: ipa-9r, tyrS1, tyrT

Categories containing this gene/protein

• translation

This gene is a member of the following regulons

• DtrR regulon, T-box

Gene

Phenotypes of a mutant

• sensitive against D-Tyr PubMed
• a tyrS tyrZ double mutant is not viable PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot) ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot)
• TyrZ is highly specific for L-Tyr, prevents misincorporation of D-Tyr into proteins, less processive than TyrS PubMed

Protein family

• TyrS type 2 subfamily (according to Swiss-Prot), class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)

Modification

• phosphorylated on Thr-311 PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operon

• dtrR-tyrZ PubMed

Sigma factor

• tyrZ PubMed

Regulation

• induced by tyrosine limitation (T-box) PubMed

Regulatory mechanism

• T-box: RNA switch, transcriptional antitermination PubMed
• DtrR: transcriptional repression PubMed

Biological materials

Mutant

• QB53532 (aphA3), available in Jörg Stülke's lab

References

Reviews

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Original publications

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