ysdA

unknown

Locus: BSU_28840

Isoelectric point: 9.96

Molecular weight: 10.18 kDa

Protein length: 89 aa

Gene length: 270 bp

Function: unknown

Product: unknown

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Groups of genes → Membrane proteins
• Groups of genes → Proteins of unknown function

Gene

Coordinates: 2,951,898 2,952,167

The protein

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: infC-rpmI-rplT-ysdA
  Description: PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response
  • RplT: termination, via binding to a RNA switch in the infC leader region causes transcription termination, in RplT regulon

  Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed

  Additional information

  • autorepression of infC-rpmI-rplT-ysdA expression upon binding of excess RplT to the untranslated region of the mRNA PubMed
  • expression of IF3 is uncoupled from that of L35 and L20 by RNase Y-dependent mRNA processing PubMed
  • the RNA switch RNA is degraded by RNase Y PubMed

Biological materials

Mutant

• GP2391 ∆ysdA::kan, available in Jörg Stülke's lab
• MGNA-B024 (ysdA::erm), available at the NBRP B. subtilis, Japan
• BKE28840 (ysdA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTGCCTCATCCTTTAT, downstream forward: _UP4_GATTTATAAAAAGCAGAAAA
• BKK28840 (ysdA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTGCCTCATCCTTTAT, downstream forward: _UP4_GATTTATAAAAAGCAGAAAA

References

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