yvdR [2019-07-10 12:03:48]

similar to multidrug-efflux transporter

locus: BSU34500

pI: 9.06

mw: 11.29 kDa

protein length: 106 aa

gene length: 321 bp

function: unknown

product: subunit of unidentified multidrug transporter

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.14|Multidrug exporters]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.3|Chaperone/ protein folding/ based on similarity]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,541,823 3,542,143

The protein

Protein family

• paired small multidrug resistance protein family ([SW|PSMR family]) [Pubmed|17942072]
• [SW|drug/metabolite transporter (DMT) superfamily] (according to UniProt)

Structure

• [PDB|3B5D] (E. coli EmrE, 33% identity) [pubmed|18024586]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|8417C205E756C27D25BCEA9394DEB0968B5F4F7B|yvdT]-[gene|FDE9E1E86BA06B4CE782CBA32F113D302A0B2231|yvdS]-[gene|18CBED273AD7787971073117DFA0B89BDF149B25|yvdR]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B618 (yvdR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1617 NBRP B. subtilis, Japan]
• BKE34500 ([gene|18CBED273AD7787971073117DFA0B89BDF149B25|yvdR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTTTTCACCTCCCGG, downstream forward: _UP4_TAAAATCAAGGCATATACAG
• BKK34500 ([gene|18CBED273AD7787971073117DFA0B89BDF149B25|yvdR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTTTTCACCTCCCGG, downstream forward: _UP4_TAAAATCAAGGCATATACAG

References

Research papers

• 22978536,18024586