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bshA [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]
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bshA [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

L-malic acid glycosyltransferase, involved in bacillithiol synthesis
locus
BSU22460
pI
6.00
mw
41.00 kDa
function
biosynthesis of bacillithiol
product
L-malic acid glycosyltransferase
essential
no
synonyms
jojH, ypjH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.2|Biosynthesis of bacillithiol]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

    • [SW|Categories] containing this gene/protein

  • [SW|miscellaneous metabolic pathways], [SW|resistance against oxidative and electrophile stress]

    • This gene is a member of the following [SW|regulons]

  • [SW|Spx regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,357,078 -> 2,358,211

    Phenotypes of a mutant

  • impaired growth in minimal medium, reduced activity of enzymes containing FeS cluster [Pubmed|25988368]
  • sensitivity to superoxide (paraquate) stress [Pubmed|25988368]

    • The protein

    Catalyzed reaction/ biological activity

  • UDP-GlcNAc L-malate = GlcNAc(α1→2)L-malate UDP [Pubmed|20308541]; also uses D-malate as a substrate, but with much lower affinity [Pubmed|20308541]

    • Protein family

  • NamA subfamily (according to Swiss-Prot)

    • Effectors of protein activity

  • subject to feedback inhibition by bacillithiol [Pubmed|22569254]

    • Structure

  • [PDB|5D00] [Pubmed|27454321]

    • Expression and Regulation

    Operon

  • ''[protein|search|ypjD]-[protein|search|dapB]-[protein|search|mgsA]-[SW|bshB1]-[protein|search|bshA]-[protein|search|cca]-[protein|search|birA]'' [Pubmed|20308541]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|23894131]

    • Regulation

  • induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]

    • Regulatory mechanism

  • [protein|search|Spx]: transcription activation [Pubmed|23894131]

    • Biological materials

    Mutant

  • ''bshA::mls'' available in [SW|John_Helmann] lab
  • GP88 (''bshA''::''pX2''(''cat'')), available in [SW|Jörg Stülke]'s lab
  • BKE22460 (''bshA''::''erm trpC2'') available from the Bacillus Genetic Stock Center

    • References

  • 20308541,19578333,22569254,20799687,23894131,25988368,27454321