yckA [2019-09-03 09:33:50]

similar to amino acid [SW|ABC transporter] (permease)

locus: BSU_03370

pI: 9.43

mw: 25.08 kDa

protein length: 226 aa

gene length: 681 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.4|Unknown ABC transporters]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 367,305 367,985

The protein

Protein family

• [SW|Binding-protein-dependent transport system permease family] (according to UniProt)

Paralogous protein(s)

• [protein|9400DF25487461E3738DDDC72BDDB1589281409D|TcyB], [protein|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|YxeN]

Structure

• [PDB|4YMS] (amino acid transporter from Caldanaerobacter subterraneus, 35% identity) [pubmed|25848002]

[SW|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|7050A2CC4BA2183C3CD22A246846CBCE43D73ABC|yckB]-[gene|1872614A6F7DBF7231348AC3DAE4ED3295ACEB41|yckA]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-C001 (yckA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1999 NBRP B. subtilis, Japan]
• BKE03370 ([gene|1872614A6F7DBF7231348AC3DAE4ED3295ACEB41|yckA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATGCTATTGATCATGGCGT, downstream forward: _UP4_TAAACAAACGTTTCATTAAA
• BKK03370 ([gene|1872614A6F7DBF7231348AC3DAE4ED3295ACEB41|yckA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATGCTATTGATCATGGCGT, downstream forward: _UP4_TAAACAAACGTTTCATTAAA

References

• 10092453,25848002