dapF [2019-08-09 09:33:00]

diaminopimelate epimerase

locus: BSU_32170

pI: 4.51

mw: 30.72 kDa

protein length: 284 aa

gene length: 855 bp

function: biosynthesis of lysine and peptidoglycan

product: diaminopimelate epimerase

essential: yes

ec: 5.1.1.7

synonyms: yutL

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.6|Biosynthesis/ acquisition of lysine/ threonine]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 3,306,040 3,306,894

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• LL-diaminopimelate - meso-diaminopimelate

Protein family

• diaminopimelate epimerase family (single member, according to UniProt)

Structure

• [PDB|2OTN] (from ''Bacillus anthracis str. ames'', 62% identity, 75% similarity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|185B6596F0468B76BCA85E8753063FAE83445E18|dapF]-[gene|94A3B5EF4ADCFA57A204B584B6D305A62E3E15AE|sufA]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B574 (yutL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1573 NBRP B. subtilis, Japan]