ypeP

similar to RNase HI

locus: BSU_21970

pI: 5.24

mw: 13.58 kDa

protein length: 226 aa

gene length: 681 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.5|RNase/ based on similarity]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.8|Not yet assigned]

Gene

Coordinates: 2,308,967 2,309,647

The protein

[SW|Domains]

• RNase H domain (aa 71-207) (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|1852AA40C48859202392834A3E791F6877DD3D30|ypeP]-[gene|A4B5C9A4BA04B0501766DADF19A49B67F92857C3|ypeQ]
  description: [pubmed|22383849]
  regulation: expressed during [SW|sporulation] [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A893 (ypeP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/893 NBRP B. subtilis, Japan]
• BP443 (''ypeP::tet'') available in [SW|Fabian Commichau]'s lab
• BKE21970 ([gene|1852AA40C48859202392834A3E791F6877DD3D30|ypeP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE21970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACAATCACCTTACCAT, downstream forward: _UP4_TTAGACCGTAACGGAGATGA
• BKK21970 ([gene|1852AA40C48859202392834A3E791F6877DD3D30|ypeP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK21970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACAATCACCTTACCAT, downstream forward: _UP4_TTAGACCGTAACGGAGATGA

Expression vectors

• for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], based on [SW|pGP380], expression from plasmid: pBP501 (E. coli amp & B. subtilis E/L) , available in [SW|Fabian Commichau]'s lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab

References

Research papers

• 29084857