yoaC [2019-06-26 09:34:03]

similar to xylulokinase

locus: BSU18550

pI: 4.86

mw: 53.94 kDa

protein length: 487 aa

gene length: 1464 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 2,022,561 2,024,024

The protein

Structure

• [PDB|2W41] (glycerol kinase from Plasmodium falciparum, 25% identity) [pubmed|19040641]

Expression and Regulation

Operons

• genes: [gene|3769B79A180412C0FE98B3FC54BA49750BAFEDF6|yoaD]-[gene|182B8EFA41DAE695B008CBAA561F3730D11750B1|yoaC]-[gene|FF60633F2902E4CA58B34FF3DAFF85B0D4007622|yoaB]
  description: [Pubmed|10094622]

  regulatory mechanism

  • [regulon|S-box|S-box]: termination, the [SW|S-box] [SW|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622], in [regulon|S-box|S-box]

  regulation

  • induced by methionine starvation ([SW|S-box]) [Pubmed|10094622]
  • the [SW|S-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

Biological materials

Mutant

• MGNA-A834 (yoaC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/834 NBRP B. subtilis, Japan]
• BKE18550 ([gene|182B8EFA41DAE695B008CBAA561F3730D11750B1|yoaC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATTCTGTTAGCCTCCT, downstream forward: _UP4_TAAGACGGAATTTCTGCTGT
• BKK18550 ([gene|182B8EFA41DAE695B008CBAA561F3730D11750B1|yoaC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATTCTGTTAGCCTCCT, downstream forward: _UP4_TAAGACGGAATTTCTGCTGT

References

• 12107147,18039762,19040641