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araM [2019-03-30 19:19:54]
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araM [2019-03-30 19:19:54]

glycerol-1-phosphate dehydrogenase, L-arabinose operon
Locus
BSU28760
Isoelectric point
5.63
Molecular weight
42.90 kDa
Protein length
394 aa Sequence Blast
Gene length
1185 bp Sequence Blast
Function
biosynthesis of phosphoglycerolipids
Product
glycerol-1-phosphate dehydrogenase
Essential
no
E.C.
1.1.1.261
Synonyms
yseB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Groups of genes → Poorly characterized/ putative enzymes

    • Gene

    Coordinates
    2,943,006 2,944,190

    The protein

    Catalyzed reaction/ biological activity

  • sn-glycerol-1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H (according to Swiss-Prot)

    • Protein family

  • glycerol-1-phosphate dehydrogenase family (according to Swiss-Prot)

    • Localization

  • cytoplasm (according to UniProt)

    • Expression and Regulation

    Operons

    Genes
    araA-araB-araD-araL-araM-araN-araP-araQ-abfA
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • AraR: repression, PubMed, in AraR regulon

    • Regulation

  • induced by arabinose (AraR) PubMed

    • Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed
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    Biological materials

    Mutant

  • MGNA-A985 (araM::erm), available at the NBRP B. subtilis, Japan
  • BKE28760 (araM::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTGAACGTCAGCTGCGATAC, downstream forward: _UP4_TAGCCCGCACCTCGAATGGA
  • BKK28760 (araM::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTGAACGTCAGCTGCGATAC, downstream forward: _UP4_TAGCCCGCACCTCGAATGGA

    • References

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