cspB [2018-06-07 19:23:39]

major cold-shock protein

locus: BSU09100

pI: 4.34

mw: 7.23 kDa

protein length: 67 aa

gene length: 201 bp

function: RNA chaperone

product: major cold-shock protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.2|RNA chaperones]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.5|Cold stress proteins]

Gene

Coordinates: 984,262 → 984,465

The protein

Paralogous protein(s)

• [protein|D453E1E7D4256B56AFB2149731E08B104D59431B|CspC], [protein|0F89D0B03F54B283CDD99A940D43C18E6B5703BB|CspD]

Structure

• [PDB|2ES2] [Pubmed|16780871]
• [PDB|1CSP] [Pubmed|8321288]
• [PDB|1NMG] (NMR)
• [PDB|3PF4] (in complex with r(GUCUUUA)) [Pubmed|22128343]

[SW|Localization]

• cytoplasm (according to Swiss-Prot), cytoplasma, colocalizes with the ribosomes [Pubmed|16352840]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]
• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|16E048FE5AF76CC771DEBBA8CB56B75A5414B3A2|cspB]
  description: [Pubmed|1400185]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1400185], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulation

  • induced upon cold shock [Pubmed|12399512]

Biological materials

Mutant

• BKE09100 (Δ[gene|16E048FE5AF76CC771DEBBA8CB56B75A5414B3A2|cspB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAAATTTCCTCCTAAAG, downstream forward: _UP4_TAAGCATAAATTGATATGAA
• BKK09100 (Δ[gene|16E048FE5AF76CC771DEBBA8CB56B75A5414B3A2|cspB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAAATTTCCTCCTAAAG, downstream forward: _UP4_TAAGCATAAATTGATATGAA

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in [SW|Stülke] lab

Labs working on this gene/protein

• [SW|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]

References

• 12399512,12171653,11752341,11717297,12838604,8321289,9533624,9379903,8294017,8755892,9914312,11591689,7476164,1409560,16352840,9920884,1400185,12427936,8307174,7703860,22128343,23199363,15378759,21124848,16780871,26809452,8321288