cspB [2019-11-27 09:04:26]

major cold-shock protein

Locus: BSU_09100

Isoelectric point: 4.34

Molecular weight: 7.23 kDa

Protein length: 67 aa

Gene length: 204 bp

Function: RNA chaperone

Product: major cold-shock protein

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → RNA synthesis and degradation → RNA chaperones
• Lifestyles → Coping with stress → Cold stress proteins

Gene

Coordinates: 984,262 984,465

The protein

Paralogous protein(s)

• CspC, CspD

Domains

• CSD domain (aa 4-63) (according to UniProt)

Structure

• 2I5M PubMed
• 2ES2 PubMed
• 1CSP PubMed
• 1NMG (NMR)
• 3PF4 (in complex with r(GUCUUUA)) PubMed

Localization

• cytoplasm (according to Swiss-Prot), cytoplasma, colocalizes with the ribosomes PubMed

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed
• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: cspB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulation

  • induced upon cold shock PubMed

Biological materials

Mutant

• GP1968 (ΔcspB::cat trpC2) available in Jörg Stülke's lab
• BKE09100 (ΔcspB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAAATTTCCTCCTAAAG, downstream forward: _UP4_TAAGCATAAATTGATATGAA
• BKK09100 (ΔcspB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAAATTTCCTCCTAAAG, downstream forward: _UP4_TAAGCATAAATTGATATGAA

Expression vector

• Constitutive overexpression of Strep-CspB with pGP3125 (in pGP380), available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

Labs working on this gene/protein

• Mohamed Marahiel, Marburg University, Germany homepage

References

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